PRRC2A sirna

PRRC2A siRNA (Human)

Cat. Num. AAA8238699

• Gene Names: PRRC2A; G2; BAT2; D6S51; D6S51E
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRRC2A; PRRC2A siRNA (Human); BAT2; G2; Protein PRRC2A; HLA-B-associated transcript 2; Large proline-rich protein BAT2; Proline-rich and coiled-coil-containing protein 2A; Protein G2; PRRC2A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRRC2A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2157

• Applicable Applications for PRRC2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRRC2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRRC2A sirna

siRNA to inhibit PRRC2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 314122241
• NCBI GeneID: 7916
• NCBI Accession #: NP_004629.3
• NCBI GenBank Nucleotide #: NM_004638.3
• UniProt Accession #: P48634; O95875; Q05BK4; Q4LE37; Q5SQ29; Q5SQ30; Q5ST84; Q5STX6; Q5STX7; B0UX77; B0UZE9; B0UZL3
• Molecular Weight: 163,047 Da
• NCBI Official Full Name: protein PRRC2A
• NCBI Official Synonym Full Names: proline-rich coiled-coil 2A
• NCBI Official Symbol: PRRC2A
• NCBI Official Synonym Symbols: G2; BAT2; D6S51; D6S51E
• NCBI Protein Information: protein PRRC2A
• UniProt Protein Name: Protein PRRC2A
• Protein Family: Protein
• UniProt Gene Name: PRRC2A
• UniProt Synonym Gene Names: BAT2; G2
• UniProt Entry Name: PRC2A_HUMAN

NCBI Description

A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. This gene has microsatellite repeats which are associated with the age-at-onset of insulin-dependent diabetes mellitus (IDDM) and possibly thought to be involved with the inflammatory process of pancreatic beta-cell destruction during the development of IDDM. This gene is also a candidate gene for the development of rheumatoid arthritis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

BAT2 iso1: a small nuclear ribonucleoprotein involved in the regulation of pre-mRNA splicing. Binds, along with the a' protein, stem loop IV of U2 snRNA. 3 isoforms of the human protein are produced by alternative splicing. Associates with snrnp u2 SNRNP u2.

Protein type: RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; nucleus

Molecular Function: protein binding