PRKAR2B sirna

PRKAR2B siRNA (Human)

Cat. Num. AAA8223000

• Gene Names: PRKAR2B; PRKAR2; RII-BETA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRKAR2B; PRKAR2B siRNA (Human); cAMP-dependent protein kinase type II-beta regulatory subunit; PRKAR2B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRKAR2B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 418

• Applicable Applications for PRKAR2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRKAR2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRKAR2B sirna

siRNA to inhibit PRKAR2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 47132585
• NCBI GeneID: 5577
• NCBI Accession #: NP_002727.2
• NCBI GenBank Nucleotide #: NM_002736.2
• UniProt Accession #: P31323; A4D0R9
• Molecular Weight: 46,302 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type II-beta regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type II, beta
• NCBI Official Symbol: PRKAR2B
• NCBI Official Synonym Symbols: PRKAR2; RII-BETA
• NCBI Protein Information: cAMP-dependent protein kinase type II-beta regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type II-beta regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKAR2B
• UniProt Entry Name: KAP3_HUMAN

NCBI Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq, Jul 2008]

Uniprot Description

PKAR2B: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Protein type: Protein kinase, regulatory subunit; Mitochondrial

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: centrosome; cytoplasm; dendritic spine; plasma membrane; dendritic shaft; cytosol; cAMP-dependent protein kinase complex

Molecular Function: ubiquitin protein ligase binding; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; organelle organization and biogenesis; activation of protein kinase A; learning; fatty acid metabolic process; signal transduction; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; mitotic cell cycle; blood coagulation; G2/M transition of mitotic cell cycle; transmembrane transport; regulation of insulin secretion