PRDM2 sirna

PRDM2 siRNA (Human)

Cat. Num. AAA8210777

• Gene Names: PRDM2; RIZ; KMT8; RIZ1; RIZ2; MTB-ZF; HUMHOXY1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRDM2; PRDM2 siRNA (Human); KMT8; RIZ; PR domain zinc finger protein 2; GATA-3-binding protein G3B; Lysine N-methyltransferase 8; MTB-ZF; MTE-binding protein; PR domain-containing protein 2; Retinoblastoma protein-interacting zinc finger protein; Zinc finger protein RIZ; PRDM2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRDM2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1481

• Applicable Applications for PRDM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRDM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRDM2 sirna

siRNA to inhibit PRDM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 55953110
• NCBI GeneID: 7799
• NCBI Accession #: NP_001007258.1
• NCBI GenBank Nucleotide #: NM_001007257.2
• UniProt Accession #: Q13029; Q13149; Q14550; Q5THJ1; Q5VUL9; B1AJZ4; B5MC68
• Molecular Weight: 162,107 Da
• NCBI Official Full Name: PR domain zinc finger protein 2 isoform c
• NCBI Official Synonym Full Names: PR domain containing 2, with ZNF domain
• NCBI Official Symbol: PRDM2
• NCBI Official Synonym Symbols: RIZ; KMT8; RIZ1; RIZ2; MTB-ZF; HUMHOXY1
• NCBI Protein Information: PR domain zinc finger protein 2
• UniProt Protein Name: PR domain zinc finger protein 2
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: PRDM2
• UniProt Synonym Gene Names: KMT8; RIZ
• UniProt Entry Name: PRDM2_HUMAN

NCBI Description

This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

PRDM2: S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; C2H2-type zinc finger protein; Transcription factor; Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.43; DNA-binding

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: Golgi apparatus; nucleus

Molecular Function: DNA binding; zinc ion binding; transcription factor activity; histone-lysine N-methyltransferase activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent