PRDM1 sirna

PRDM1 siRNA (Human)

Cat. Num. AAA8227348

• Gene Names: PRDM1; BLIMP1; PRDI-BF1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRDM1; PRDM1 siRNA (Human); BLIMP1; PR domain zinc finger protein 1; BLIMP-1; Beta-interferon gene positive regulatory domain I-binding factor; PR domain-containing protein 1; Positive regulatory domain I-binding factor 1; PRDI-BF1; PRDI-binding factor 1; PRDM1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRDM1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 691

• Applicable Applications for PRDM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRDM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRDM1 sirna

siRNA to inhibit PRDM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 172072684
• NCBI GeneID: 639
• NCBI Accession #: NP_001189.2
• NCBI GenBank Nucleotide #: NM_001198.3
• UniProt Accession #: O75626; Q86WM7; B2REA6; E1P5E0
• Molecular Weight: 76,835 Da
• NCBI Official Full Name: PR domain zinc finger protein 1 isoform 1
• NCBI Official Synonym Full Names: PR domain containing 1, with ZNF domain
• NCBI Official Symbol: PRDM1
• NCBI Official Synonym Symbols: BLIMP1; PRDI-BF1
• NCBI Protein Information: PR domain zinc finger protein 1
• UniProt Protein Name: PR domain zinc finger protein 1
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: PRDM1
• UniProt Synonym Gene Names: BLIMP1; PRDI-BF1; PRDI-binding factor 1
• UniProt Entry Name: PRDM1_HUMAN

NCBI Description

This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDM1: a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene and can inhibit virus-mediated IFN-¿ production. Recruits chromatin-modifying enzymes including histone deacetylases and methyltransferases. Drives the maturation of B-lymphocytes into Ig secreting cells. Interacts with PRMT5. Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1. In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation and in a subset of T-cells suggesting that it may play a wider role in homeostasis and differentiation. Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc, CIITA, Pax5, Spi-B, and Id3.Three isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription, coactivator/corepressor; Transcription regulation; Methyltransferase, protein lysine, predicted; DNA-binding

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; nucleus

Molecular Function: methyltransferase activity; protein binding; histone deacetylase binding; metal ion binding; transcription factor activity

Biological Process: methylation; negative regulation of lipopolysaccharide-mediated signaling pathway; eye photoreceptor cell development; cell fate commitment; transcription, DNA-dependent; positive regulation of B cell differentiation; negative regulation of B cell proliferation; negative regulation of transcription from RNA polymerase II promoter; maternal placenta development; germ cell development; post-embryonic development