PPIP5K1 sirna

PPIP5K1 siRNA (Human)

Cat. Num. AAA8207474

• Gene Names: PPIP5K1; IP6K; IPS1; VIP1; hsVIP1; HISPPD2A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPIP5K1; PPIP5K1 siRNA (Human); HISPPD2A; IP6K; IPS1; KIAA0377; VIP1; Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1; Diphosphoinositol pentakisphosphate kinase 1; Histidine acid phosphatase domain-containing protein 2A; IP6 kinase; Inositol pyrophosphate synthase 1; InsP6 and PP-IP5 kinase 1; VIP1 homolog; hsVIP1; PPIP5K1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PPIP5K1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,406

• Applicable Applications for PPIP5K1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PPIP5K1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPIP5K1 sirna

siRNA to inhibit PPIP5K1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 195947359
• NCBI GeneID: 9677
• NCBI Accession #: NP_001124330.1
• NCBI GenBank Nucleotide #: NM_001130858.2
• UniProt Accession #: Q6PFW1; O15082; Q5HYF8; Q7Z3A7; Q86TE7; Q86UV3; Q86UV4; Q86XW8; Q8IZN0
• Molecular Weight: 156,320 Da
• NCBI Official Full Name: inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1 isoform 5
• NCBI Official Synonym Full Names: diphosphoinositol pentakisphosphate kinase 1
• NCBI Official Symbol: PPIP5K1
• NCBI Official Synonym Symbols: IP6K; IPS1; VIP1; hsVIP1; HISPPD2A
• NCBI Protein Information: inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1
• UniProt Protein Name: Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1
• Protein Family: Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase
• UniProt Gene Name: PPIP5K1
• UniProt Synonym Gene Names: HISPPD2A; IP6K; IPS1; KIAA0377; VIP1; hsVIP1
• UniProt Entry Name: VIP1_HUMAN

NCBI Description

This gene encodes a dual functional inositol kinase. The encoded enzyme converts inositol hexakisphosphate to diphosphoinositol pentakisphosphate and diphosphoinositol pentakisphosphate to bis-diphosphoinositol tetrakisphosphate. This protein may be important for intracellular signaling pathways. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 15.[provided by RefSeq, Jun 2010]

Uniprot Description

PPIP5K1: a bifunctional inositol kinase that catalyzes the formation of diphosphoinositol pentakisphosphate (InsP7 or PP-InsP5) and bi-diphosphoinositol tetrakisphosphate (InsP8 or PP2-InsP4). Converts inositolitol hexakisphosphate (InsP6) to InsP7. Also able to convert InsP7 to InsP8. Probably specifically mediates the formation of 4PP-InsP5 and 6PP-InsP5 InsP7 isomers but not of 5PP-IP5 InsP7 isomer. Activated when cells are exposed to hyperosmotic stress. Belongs to the histidine acid phosphatase family. VIP1 subfamily. The His_Phos_2 domain has no apparent phosphatase activity but has been shown to have cryptic polyphosphoinositide binding activity. Seven isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.4.21; Kinase, other; Phosphatase (non-protein); EC 2.7.4.24

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: inositol 1,3,4,5,6-pentakisphosphate kinase activity; acid phosphatase activity; inositol hexakisphosphate 5-kinase activity; ATP binding; diphosphoinositol-pentakisphosphate kinase activity

Biological Process: inositol phosphate metabolic process; dephosphorylation; inositol metabolic process; phosphorylation