POU4F1 sirna

POU4F1 siRNA (Human)

Cat. Num. AAA8231809

• Gene Names: POU4F1; BRN3A; RDC-1; Oct-T1; brn-3A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POU4F1; POU4F1 siRNA (Human); BRN3A; RDC1; POU domain. class 4. transcription factor 1; Brain-specific homeobox/POU domain protein 3A; Brain-3A; Brn-3A; Homeobox/POU domain protein RDC-1; Oct-T1; POU4F1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POU4F1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 419

• Applicable Applications for POU4F1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POU4F1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POU4F1 sirna

siRNA to inhibit POU4F1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110347449
• NCBI GeneID: 5457
• NCBI Accession #: NP_006228.3
• NCBI GenBank Nucleotide #: NM_006237.3
• UniProt Accession #: Q01851; Q14986; Q15318; Q5T227
• Molecular Weight: 42,697 Da
• NCBI Official Full Name: POU domain, class 4, transcription factor 1
• NCBI Official Synonym Full Names: POU class 4 homeobox 1
• NCBI Official Symbol: POU4F1
• NCBI Official Synonym Symbols: BRN3A; RDC-1; Oct-T1; brn-3A
• NCBI Protein Information: POU domain, class 4, transcription factor 1
• UniProt Protein Name: POU domain, class 4, transcription factor 1
• Protein Family: POU domain, class 4, transcription factor
• UniProt Gene Name: POU4F1
• UniProt Synonym Gene Names: BRN3A; RDC1; Brain-3A; Brn-3A
• UniProt Entry Name: PO4F1_HUMAN

NCBI Description

This gene encodes a member of the POU-IV class of neural transcription factors. This protein is expressed in a subset of retinal ganglion cells and may be involved in the developing sensory nervous system. This protein may also promote the growth of cervical tumors. A translocation of this gene is associated with some adult acute myeloid leukemias. [provided by RefSeq, Mar 2012]

Uniprot Description

POU4F1: Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: Cell development/differentiation; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 13q31.1

Cellular Component: neuron projection; nuclear chromatin

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding

Biological Process: transcription from RNA polymerase II promoter; central nervous system neuron differentiation; trigeminal nerve development; positive regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; habenula development; cell migration in hindbrain; peripheral nervous system neuron development; proprioception during equilibrioception; synaptogenesis; regulation of neurogenesis; suckling behavior; axonogenesis; neuron fate specification; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; sensory system development; negative regulation of neuron apoptosis