PNCK sirna
PNCK siRNA (Human)
Gene Names
PNCK; BSTK3; CaMK1b
Reactivity
Human
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
PNCK; PNCK siRNA (Human); Calcium/calmodulin-dependent protein kinase type 1B; CaM kinase I beta; CaM kinase IB; CaM-KI beta; CaMKI-beta; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase; PNCK sirna
Host
Synthetic
Reactivity
Human
Specificity
PNCK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
426
Applicable Applications for PNCK sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PNCK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for PNCK sirna
siRNA to inhibit PNCK expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,356 Da
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type 1B isoform a
NCBI Official Synonym Full Names
pregnancy up-regulated nonubiquitous CaM kinase
NCBI Official Symbol
PNCK
NCBI Official Synonym Symbols
BSTK3; CaMK1b
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type 1B
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type 1B
Protein Family
UniProt Gene Name
PNCK
UniProt Synonym Gene Names
CaM kinase IB; CaM-KI beta; CaMKI-beta
UniProt Entry Name
KCC1B_HUMAN
NCBI Description
PNCK is a member of the calcium/calmodulin-dependent protein kinase family of protein serine/threonine kinases (see CAMK1; MIM 604998) (Gardner et al., 2000 [PubMed 10673339]).[supplied by OMIM, Mar 2008]
Uniprot Description
CAMK1B: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.17; CAMK group; CAMK1 family
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: cytoplasm; nucleus
Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding
Biological Process: protein amino acid phosphorylation
Research Articles on PNCK
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Product Notes
The PNCK pnck (Catalog #AAA8208866) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The PNCK siRNA (Human) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PNCK can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the PNCK pnck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNCK, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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