PLTP sirna

PLTP siRNA (Human)

Cat. Num. AAA8212902

• Gene Names: PLTP; BPIFE; HDLCQ9
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLTP; PLTP siRNA (Human); Phospholipid transfer protein; Lipid transfer protein II; PLTP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PLTP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 398

• Applicable Applications for PLTP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PLTP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLTP sirna

siRNA to inhibit PLTP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 339275807
• NCBI GeneID: 5360
• NCBI Accession #: NP_001229849.1
• NCBI GenBank Nucleotide #: NM_001242920.1
• UniProt Accession #: P55058; Q8WTT1; Q9BR07; Q9BSH8; A8K006; B4DDD5; B4DRB4; E1P5N8; E7EV16
• Molecular Weight: 44,847 Da
• NCBI Official Full Name: phospholipid transfer protein isoform c
• NCBI Official Synonym Full Names: phospholipid transfer protein
• NCBI Official Symbol: PLTP
• NCBI Official Synonym Symbols: BPIFE; HDLCQ9
• NCBI Protein Information: phospholipid transfer protein
• UniProt Protein Name: Phospholipid transfer protein
• Protein Family: Phospholipid transfer protein
• UniProt Gene Name: PLTP
• UniProt Entry Name: PLTP_HUMAN

NCBI Description

The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PLTP: Converts HDL into larger and smaller particles. May play a key role in extracellular phospholipid transport and modulation of hdl particles. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Lipid-binding; Secreted

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space; extracellular region

Molecular Function: lipid binding

Biological Process: sperm motility; vitamin E biosynthetic process; lipid metabolic process; lipid transport