PLS3 sirna

PLS3 siRNA (Human)

Cat. Num. AAA8229884

• Gene Names: PLS3; BMND18; T-plastin
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLS3; PLS3 siRNA (Human); Plastin-3; T-plastin; PLS3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PLS3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 630

• Applicable Applications for PLS3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PLS3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLS3 sirna

siRNA to inhibit PLS3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209862851
• NCBI GeneID: 5358
• NCBI Accession #: NP_001129497.1
• NCBI GenBank Nucleotide #: NM_001136025.4
• UniProt Accession #: P13797; Q86YI6; A8K579; B1AQ09; B4DGB4; B7Z6M1
• Molecular Weight: 65,632 Da
• NCBI Official Full Name: plastin-3 isoform 1
• NCBI Official Synonym Full Names: plastin 3
• NCBI Official Symbol: PLS3
• NCBI Official Synonym Symbols: BMND18; T-plastin
• NCBI Protein Information: plastin-3
• UniProt Protein Name: Plastin-3
• Protein Family: Plastin
• UniProt Gene Name: PLS3
• UniProt Entry Name: PLST_HUMAN

NCBI Description

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

PLS3: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: cytoplasm

Molecular Function: calcium ion binding; actin binding

Disease: Bone Mineral Density Quantitative Trait Locus 18