PLA2R1 sirna

PLA2R1 siRNA (Human)

Cat. Num. AAA8222698

• Gene Names: PLA2R1; PLA2R; PLA2-R; PLA2IR; CLEC13C; PLA2G1R
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLA2R1; PLA2R1 siRNA (Human); CLEC13C; Secretory phospholipase A2 receptor; PLA2-R; PLA2R; 180 kDa secretory phospholipase A2 receptor; C-type lectin domain family 13 member C; M-type receptor; PLA2R1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PLA2R1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1324

• Applicable Applications for PLA2R1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PLA2R1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLA2R1 sirna

siRNA to inhibit PLA2R1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 55953106
• NCBI GeneID: 22925
• NCBI Accession #: NP_001007268.1
• NCBI GenBank Nucleotide #: NM_001007267.2
• UniProt Accession #: Q13018; Q13019; Q15095; Q53R45; Q53RR7; B2RTU9; D3DPB1
• Molecular Weight: 152,805 Da
• NCBI Official Full Name: secretory phospholipase A2 receptor isoform 2
• NCBI Official Synonym Full Names: phospholipase A2 receptor 1, 180kDa
• NCBI Official Symbol: PLA2R1
• NCBI Official Synonym Symbols: PLA2R; PLA2-R; PLA2IR; CLEC13C; PLA2G1R
• NCBI Protein Information: secretory phospholipase A2 receptor
• UniProt Protein Name: Secretory phospholipase A2 receptor
• Protein Family: Secretory phospholipase A2 receptor
• UniProt Gene Name: PLA2R1
• UniProt Synonym Gene Names: CLEC13C; PLA2-R; PLA2R; Soluble PLA2-R; Soluble PLA2R
• UniProt Entry Name: PLA2R_HUMAN

NCBI Description

This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

Uniprot Description

PLA2R1: Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2- IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2- IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow- derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q23-q24

Cellular Component: cell surface; integral to plasma membrane; integral to membrane; plasma membrane; extracellular region; receptor complex

Molecular Function: phospholipase binding; receptor activity; carbohydrate binding

Biological Process: receptor-mediated endocytosis; cytokine production; endocytosis; positive regulation of DNA damage response, signal transduction by p53 class mediator