PIGK sirna

PIGK siRNA (Human)

Cat. Num. AAA827468

• Gene Names: PIGK; GPI8
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIGK; PIGK siRNA (Human); GPI8; GPI-anchor transamidase; GPI transamidase; GPI8 homolog; hGPI8; Phosphatidylinositol-glycan biosynthesis class K protein; PIG-K; PIGK sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PIGK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 395

• Applicable Applications for PIGK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PIGK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIGK sirna

siRNA to inhibit PIGK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 23199983
• NCBI GeneID: 10026
• NCBI Accession #: NP_005473.1
• NCBI GenBank Nucleotide #: NM_005482.2
• UniProt Accession #: Q92643; O14822; Q5TG77; B2R7K3; B4E2M3
• Molecular Weight: 36,416 Da
• NCBI Official Full Name: GPI-anchor transamidase
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis, class K
• NCBI Official Symbol: PIGK
• NCBI Official Synonym Symbols: GPI8
• NCBI Protein Information: GPI-anchor transamidase
• UniProt Protein Name: GPI-anchor transamidase
• UniProt Gene Name: PIGK
• UniProt Synonym Gene Names: GPI8; GPI transamidase; hGPI8; PIG-K
• UniProt Entry Name: GPI8_HUMAN

NCBI Description

This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

PIGK: Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein. Belongs to the peptidase C13 family.

Protein type: EC 3.-.-.-; Protease; Endoplasmic reticulum; Membrane protein, integral; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: endoplasmic reticulum membrane; membrane; integral to endoplasmic reticulum membrane; GPI-anchor transamidase complex

Molecular Function: protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; GPI-anchor transamidase activity

Biological Process: cellular protein metabolic process; protein folding; attachment of GPI anchor to protein; C-terminal protein lipidation; proteolysis; post-translational protein modification