PIAS3 sirna

PIAS3 siRNA (Human)

Cat. Num. AAA8227686

• Gene Names: PIAS3; ZMIZ5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIAS3; PIAS3 siRNA (Human); E3 SUMO-protein ligase PIAS3; Protein inhibitor of activated STAT protein 3; PIAS3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PIAS3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 628

• Applicable Applications for PIAS3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PIAS3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIAS3 sirna

siRNA to inhibit PIAS3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 115298686
• NCBI GeneID: 10401
• NCBI Accession #: NP_006090.2
• NCBI GenBank Nucleotide #: NM_006099.3
• UniProt Accession #: Q9Y6X2; Q9UFI3
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS3
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT, 3
• NCBI Official Symbol: PIAS3
• NCBI Official Synonym Symbols: ZMIZ5
• NCBI Protein Information: E3 SUMO-protein ligase PIAS3
• UniProt Protein Name: E3 SUMO-protein ligase PIAS3
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: PIAS3
• UniProt Entry Name: PIAS3_HUMAN

NCBI Description

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PIAS3: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Monomer. Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3- mediated transcriptional activity. Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. By dihydrotestosterone (DHT) in prostate cancer cells. Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. Belongs to the PIAS family.

Protein type: SUMO conjugating system; Nuclear receptor co-regulator; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: dendrite; cytoplasm; synapse; nucleus

Molecular Function: protein C-terminus binding; protein binding; potassium channel regulator activity; enzyme binding; zinc ion binding; protein N-terminus binding; SUMO ligase activity; ligase activity

Biological Process: positive regulation of protein sumoylation; protein sumoylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to hormone stimulus; positive regulation of membrane potential