PEX10 sirna

PEX10 siRNA (Human)

Cat. Num. AAA8233803

• Gene Names: PEX10; NALD; PBD6A; PBD6B; RNF69
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PEX10; PEX10 siRNA (Human); RNF69; Peroxisome biogenesis factor 10; Peroxin-10; Peroxisomal biogenesis factor 10; Peroxisome assembly protein 10; RING finger protein 69; PEX10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PEX10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 326

• Applicable Applications for PEX10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PEX10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PEX10 sirna

siRNA to inhibit PEX10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4505715
• NCBI GeneID: 5192
• NCBI Accession #: NP_002608.1
• NCBI GenBank Nucleotide #: NM_002617.3
• UniProt Accession #: O60683; Q5T095; Q9BW90; B3KWD8
• Molecular Weight: 39,214 Da
• NCBI Official Full Name: peroxisome biogenesis factor 10 isoform 2
• NCBI Official Synonym Full Names: peroxisomal biogenesis factor 10
• NCBI Official Symbol: PEX10
• NCBI Official Synonym Symbols: NALD; PBD6A; PBD6B; RNF69
• NCBI Protein Information: peroxisome biogenesis factor 10
• UniProt Protein Name: Peroxisome biogenesis factor 10
• Protein Family: Peroxisome biogenesis factor
• UniProt Gene Name: PEX10
• UniProt Synonym Gene Names: RNF69
• UniProt Entry Name: PEX10_HUMAN

NCBI Description

This gene encodes a protein involved in import of peroxisomal matrix proteins. This protein localizes to the peroxisomal membrane. Mutations in this gene result in phenotypes within the Zellweger spectrum of peroxisomal biogenesis disorders, ranging from neonatal adrenoleukodystrophy to Zellweger syndrome. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PEX10: Somewhat implicated in the biogenesis of peroxisomes. Defects in PEX10 are the cause of peroxisome biogenesis disorder complementation group 7 (PBD-CG7); also known as PBD-CGB. PBD refers to a group of peroxisomal disorders arising from a failure of protein import into the peroxisomal membrane or matrix. The PBD group is comprised of four disorders: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum. The PBD group is genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies. Defects in PEX10 are a cause of Zellweger syndrome (ZWS). ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life. Defects in PEX10 are a cause of adrenoleukodystrophy neonatal (NALD). NALD is a peroxisome biogenesis disorder (PBD) characterized by the accumulation of very long- chain fatty acids, adrenal insufficiency and mental retardation. Belongs to the pex2/pex10/pex12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 1p36.32

Cellular Component: peroxisomal membrane; integral to peroxisomal membrane; peroxisome; intracellular

Molecular Function: protein C-terminus binding; protein binding; zinc ion binding

Biological Process: peroxisome organization and biogenesis; protein import into peroxisome matrix

Disease: Peroxisome Biogenesis Disorder 6b; Peroxisome Biogenesis Disorder 6a (zellweger)