PDS5B sirna

PDS5B siRNA (Human)

Cat. Num. AAA8229550

• Gene Names: PDS5B; AS3; APRIN; CG008
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDS5B; PDS5B siRNA (Human); APRIN; AS3; KIAA0979; Sister chromatid cohesion protein PDS5 homolog B; Androgen-induced proliferation inhibitor; Androgen-induced prostate proliferative shutoff-associated protein AS3; PDS5B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PDS5B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1447

• Applicable Applications for PDS5B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PDS5B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDS5B sirna

siRNA to inhibit PDS5B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7657269
• NCBI GeneID: 23047
• NCBI Accession #: NP_055847.1
• NCBI GenBank Nucleotide #: NM_015032.3
• UniProt Accession #: Q9NTI5; Q5R3S3; Q5W0K8; Q6NSC3; Q8IXT6; Q9H5N8; Q9Y2I5; Q9Y451
• Molecular Weight: 14,281 Da
• NCBI Official Full Name: sister chromatid cohesion protein PDS5 homolog B
• NCBI Official Synonym Full Names: PDS5 cohesin associated factor B
• NCBI Official Symbol: PDS5B
• NCBI Official Synonym Symbols: AS3; APRIN; CG008
• NCBI Protein Information: sister chromatid cohesion protein PDS5 homolog B
• UniProt Protein Name: Sister chromatid cohesion protein PDS5 homolog B
• Protein Family: Sister chromatid cohesion protein
• UniProt Gene Name: PDS5B
• UniProt Entry Name: PDS5B_HUMAN

NCBI Description

This gene encodes a protein that interacts with the conserved protein complex termed cohesin. The cohesin complex holds together sister chromatids and facilitates accurate chromosome segregation during mitosis and meiosis. This protein is also a negative regulator of cell proliferation and may be a tumor-suppressor gene. [provided by RefSeq, Jul 2015]

Uniprot Description

APRIN: a widely expressed nuclear protein. Is lost in many cancers and may function as a tumor suppressor. Correlates with differentiation in embryonal carcinoma stem cells; its knockdown disrupted Nanog, Oct4, and SOX2 regulation. Plays a role in androgen-induced proliferative arrest in prostate cells. Is upregulated in androgen-sensitive LNCaP prostate cancer cells undergoing androgen-induced proliferative block. Required for maintenance of sister chromatid cohesion during mitosis. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Belongs to the PDS5 family. Induced by the synthetic androgen R1881 in prostate carcinoma cells undergoing proliferative arrest. Phosphorylated upon DNA damage. Maximum levels occur 18-20 hours after androgen exposure. Interacts with the cohesin complex. Phosphorylated upon DNA damage, probably by ATM or ATR. Five isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; chromosome; nucleus; cytosol; chromatin; chromosome, pericentric region

Molecular Function: protein binding; DNA binding; ATP binding

Biological Process: cell proliferation; negative regulation of cell proliferation; cell division; mitotic sister chromatid cohesion; mitotic cell cycle; regulation of cell proliferation