PCGF2 sirna

PCGF2 siRNA (Human)

Cat. Num. AAA8225417

• Gene Names: PCGF2; MEL-18; RNF110; ZNF144
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PCGF2; PCGF2 siRNA (Human); MEL18; RNF110; ZNF144; Polycomb group RING finger protein 2; DNA-binding protein Mel-18; RING finger protein 110; Zinc finger protein 144; PCGF2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PCGF2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 344

• Applicable Applications for PCGF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PCGF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PCGF2 sirna

siRNA to inhibit PCGF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6005964
• NCBI GeneID: 7703
• NCBI Accession #: NP_009075.1
• NCBI GenBank Nucleotide #: NM_007144.2
• UniProt Accession #: P35227; A6NGD8
• Molecular Weight: 37,788 Da
• NCBI Official Full Name: polycomb group RING finger protein 2
• NCBI Official Synonym Full Names: polycomb group ring finger 2
• NCBI Official Symbol: PCGF2
• NCBI Official Synonym Symbols: MEL-18; RNF110; ZNF144
• NCBI Protein Information: polycomb group RING finger protein 2
• UniProt Protein Name: Polycomb group RING finger protein 2
• Protein Family: Polycomb group RING finger protein
• UniProt Gene Name: PCGF2
• UniProt Synonym Gene Names: MEL18; RNF110; ZNF144
• UniProt Entry Name: PCGF2_HUMAN

NCBI Description

The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses. [provided by RefSeq, Jul 2008]

Uniprot Description

PCGF2: Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero- posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with Bmi1; unlike Bmi1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex.

Protein type: Transcription factor; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: nucleoplasm; nuclear body; sex chromatin; nuclear chromatin; PcG protein complex; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; chromatin binding; transcription factor activity

Biological Process: anterior/posterior pattern formation; transcription, DNA-dependent; in utero embryonic development; protein ubiquitination; histone acetylation; negative regulation of transcription from RNA polymerase II promoter; embryonic skeletal morphogenesis