PAK3 sirna

PAK3 siRNA (Human)

Cat. Num. AAA8211190

• Gene Names: PAK3; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PAK3; PAK3 siRNA (Human); OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3; PAK3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PAK3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 565

• Applicable Applications for PAK3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PAK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PAK3 sirna

siRNA to inhibit PAK3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 189491751
• NCBI GeneID: 5063
• NCBI Accession #: NP_001121638.1
• NCBI GenBank Nucleotide #: NM_001128166.1
• UniProt Accession #: O75914; Q5JWX1; Q5JWX2; Q7Z2D6; Q7Z2E4; Q7Z3Z8; Q8WWK5; Q8WX23; A8K389; B1GX77; B1GX78; B1GX79
• Molecular Weight: 62,913 Da
• NCBI Official Full Name: serine/threonine-protein kinase PAK 3 isoform a
• NCBI Official Synonym Full Names: p21 protein (Cdc42/Rac)-activated kinase 3
• NCBI Official Symbol: PAK3
• NCBI Official Synonym Symbols: bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
• NCBI Protein Information: serine/threonine-protein kinase PAK 3
• UniProt Protein Name: Serine/threonine-protein kinase PAK 3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK3
• UniProt Synonym Gene Names: OPHN3; PAK-3
• UniProt Entry Name: PAK3_HUMAN

NCBI Description

PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

PAK3: a protein kinase of the STE20 family that regulates synapse formation and plasticity in the hippocampus. May be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Forms an activated complex with GTP-bound P21, CDC2 and RAC1 proteins. Missense and truncation mutations linked to nonsyndromic mental retardation type 30 (MRX30). Two alternatively spliced human isoforms have been reported.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: cytoplasm; plasma membrane; cytosol; endosome

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; SH3 domain binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: axon guidance; activation of MAPK activity; MAPKKK cascade; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of actin filament polymerization; axonogenesis; positive regulation of neuron apoptosis; T cell costimulation; synapse organization and biogenesis; ephrin receptor signaling pathway; dendrite development; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway

Disease: Mental Retardation, X-linked 30