P2RY12 sirna

P2RY12 siRNA (Human)

Cat. Num. AAA8238161

• Gene Names: P2RY12; HORK3; P2Y12; ADPG-R; BDPLT8; SP1999; P2T(AC); P2Y(AC); P2Y(12)R; P2Y(ADP); P2Y(cyc)
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: P2RY12; P2RY12 siRNA (Human); HORK3; P2Y purinoceptor 12; P2Y12; ADP-glucose receptor; ADPG-R; P2T(AC); P2Y(AC); P2Y(cyc); P2Y12 platelet ADP receptor; P2Y(ADP); SP1999; P2RY12 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: P2RY12 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 342

• Applicable Applications for P2RY12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human P2RY12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for P2RY12 sirna

siRNA to inhibit P2RY12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 12232483
• NCBI GeneID: 64805
• NCBI Accession #: NP_073625.1
• NCBI GenBank Nucleotide #: NM_022788.4
• UniProt Accession #: Q9H244; Q546J7; D3DNJ5
• Molecular Weight: 39,439 Da
• NCBI Official Full Name: P2Y purinoceptor 12
• NCBI Official Synonym Full Names: purinergic receptor P2Y, G-protein coupled, 12
• NCBI Official Symbol: P2RY12
• NCBI Official Synonym Symbols: HORK3; P2Y12; ADPG-R; BDPLT8; SP1999; P2T(AC); P2Y(AC); P2Y(12)R; P2Y(ADP); P2Y(cyc)
• NCBI Protein Information: P2Y purinoceptor 12
• UniProt Protein Name: P2Y purinoceptor 12
• Protein Family: P2Y purinoceptor
• UniProt Gene Name: P2RY12
• UniProt Synonym Gene Names: HORK3; P2Y12; ADPG-R; P2Y(ADP)
• UniProt Entry Name: P2Y12_HUMAN

NCBI Description

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Mutations in this gene are implicated in bleeding disorder, platelet type 8 (BDPLT8). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

P2Y12: Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation. Defects in P2RY12 are the cause of bleeding disorder platelet-type 8 (BDPLT8). A condition characterized by mild to moderate mucocutaneous bleeding, and excessive bleeding after surgery or trauma. The defect is due to severe impairment of platelet response to ADP resulting in defective platelet aggregation. Belongs to the G-protein coupled receptor 1 family.

Protein type: Mitochondrial; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: cell surface; mitochondrion; integral to plasma membrane; plasma membrane; basal plasma membrane; intrinsic to membrane; caveola; external side of plasma membrane

Molecular Function: guanyl-nucleotide exchange factor activity; adenosine receptor activity, G-protein coupled; platelet ADP receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; platelet activation; negative regulation of cell differentiation; cell projection organization and biogenesis; hemostasis; protein kinase B signaling cascade; positive regulation of ion transport; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of calcium ion transport; blood coagulation; adenosine receptor signaling pathway; positive regulation of GTPase activity

Disease: Bleeding Disorder, Platelet-type, 8