OMA1 sirna

OMA1 siRNA (Human)

Cat. Num. AAA827349

• Gene Names: OMA1; DAB1; MPRP-1; YKR087C; ZMPOMA1; peptidase; 2010001O09Rik
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: OMA1; OMA1 siRNA (Human); MPRP1; Metalloendopeptidase OMA1 mitochondrial; Metalloprotease-related protein 1; MPRP-1; Overlapping with the m-AAA protease 1 homolog; OMA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: OMA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 524

• Applicable Applications for OMA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human OMA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for OMA1 sirna

siRNA to inhibit OMA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21686999
• NCBI GeneID: 115209
• NCBI Accession #: NP_660286.1
• NCBI GenBank Nucleotide #: NM_145243.4
• UniProt Accession #: Q96E52; Q5T3G6; Q5T3G7; Q5T3G8; Q5T3G9; Q5T3H0; Q8NBB3; D3DQ54
• Molecular Weight: 55,744 Da
• NCBI Official Full Name: metalloendopeptidase OMA1, mitochondrial
• NCBI Official Synonym Full Names: OMA1 zinc metallopeptidase
• NCBI Official Symbol: OMA1
• NCBI Official Synonym Symbols: DAB1; MPRP-1; YKR087C; ZMPOMA1; peptidase; 2010001O09Rik
• NCBI Protein Information: metalloendopeptidase OMA1, mitochondrial
• UniProt Protein Name: Metalloendopeptidase OMA1, mitochondrial
• Protein Family: Metalloendopeptidase
• UniProt Gene Name: OMA1
• UniProt Synonym Gene Names: MPRP1; MPRP-1
• UniProt Entry Name: OMA1_HUMAN

Uniprot Description

OMA1: Metalloprotease that is part of the quality control system in the inner membrane of mitochondria. Following stress conditions that induce loss of mitochondrial membrane potential, mediates cleavage of OPA1 at S1 position, leading to OPA1 inactivation and negative regulation of mitochondrial fusion. Its role in mitochondrial quality control is essential for regulating lipid metabolism as well as to maintain body temperature and energy expenditure under cold-stress conditions. Belongs to the peptidase M48 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 1p32.2-p32.1

Cellular Component: mitochondrial membrane; mitochondrial inner membrane; integral to membrane

Molecular Function: metalloendopeptidase activity; metal ion binding

Biological Process: diet induced thermogenesis; cristae formation; misfolded or incompletely synthesized protein catabolic process; response to stress; glucose metabolic process; lipid metabolic process