NUP153 sirna

NUP153 siRNA (Human)

Cat. Num. AAA8209738

• Gene Names: NUP153; N153; HNUP153
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NUP153; NUP153 siRNA (Human); Nuclear pore complex protein Nup153; 153 kDa nucleoporin; Nucleoporin Nup153; NUP153 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NUP153 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1506

• Applicable Applications for NUP153 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NUP153 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NUP153 sirna

siRNA to inhibit NUP153 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 503774352
• NCBI GeneID: 9972
• NCBI Accession #: NP_001265138.1
• NCBI GenBank Nucleotide #: NM_001278209.1
• UniProt Accession #: P49790; Q4LE47; Q5T9I7; Q7Z743; B4DIK2; E7EPX5; F6QR24
• Molecular Weight: 157,338 Da
• NCBI Official Full Name: nuclear pore complex protein Nup153 isoform 1
• NCBI Official Synonym Full Names: nucleoporin 153kDa
• NCBI Official Symbol: NUP153
• NCBI Official Synonym Symbols: N153; HNUP153
• NCBI Protein Information: nuclear pore complex protein Nup153
• UniProt Protein Name: Nuclear pore complex protein Nup153
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP153
• UniProt Entry Name: NU153_HUMAN

NCBI Description

Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

NUP153: Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.

Protein type: Nucleoporin; Nuclear export

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleolus; nuclear pore; nuclear inclusion body

Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; structural constituent of nuclear pore; transporter activity; protein anchor; nucleocytoplasmic transporter activity

Biological Process: nuclear pore complex assembly; entry of virus into host cell; mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; viral infectious cycle; glucose transport; negative regulation of RNA export from nucleus; protein transport; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; transmembrane transport