NSUN2 sirna

NSUN2 siRNA (Human)

Cat. Num. AAA8222455

• Gene Names: NSUN2; MISU; MRT5; SAKI; TRM4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NSUN2; NSUN2 siRNA (Human); SAKI; TRM4; tRNA; cytosine(34)-C(5))-methyltransferase; Myc-induced SUN domain-containing protein; Misu; NOL1/NOP2/Sun domain family member 2; Substrate of AIM1/Aurora kinase B; cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolog; hTrm4; NSUN2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NSUN2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 732

• Applicable Applications for NSUN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NSUN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NSUN2 sirna

siRNA to inhibit NSUN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 301336155
• NCBI GeneID: 54888
• NCBI Accession #: NP_001180384.1
• NCBI GenBank Nucleotide #: NM_001193455.1
• UniProt Accession #: Q08J23; Q9BVN4; Q9H858; Q9NXD9; A8K529; B2RNR4; B3KP09; B4DQW2; G3V1R4
• Molecular Weight: 59,384 Da
• NCBI Official Full Name: tRNA (cytosine(34)-C(5))-methyltransferase isoform 2
• NCBI Official Synonym Full Names: NOP2/Sun RNA methyltransferase family, member 2
• NCBI Official Symbol: NSUN2
• NCBI Official Synonym Symbols: MISU; MRT5; SAKI; TRM4
• NCBI Protein Information: tRNA (cytosine(34)-C(5))-methyltransferase
• UniProt Protein Name: tRNA (cytosine(34)-C(5))-methyltransferase
• Protein Family: tRNA
• UniProt Gene Name: NSUN2
• UniProt Synonym Gene Names: SAKI; TRM4; Misu; hTrm4
• UniProt Entry Name: NSUN2_HUMAN

NCBI Description

This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NSUN2: an RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity. Interacts with NPM1 and NCL during interphase. Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA. Defects in NSUN2 are the cause of mental retardation autosomal recessive type 5 (MRT5), a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily. Two human isoforms produced by alternative splicing.

Protein type: RNA-binding; Methyltransferase; Nucleolus; EC 2.1.1.203

Chromosomal Location of Human Ortholog: 5p15.31

Cellular Component: cytoplasm; nucleolus; spindle; nucleus

Molecular Function: tRNA (cytosine-5-)-methyltransferase activity; tRNA binding

Biological Process: mitosis; cell division; tRNA methylation

Disease: Mental Retardation, Autosomal Recessive 5