NOVA1 sirna

NOVA1 siRNA (Human)

Cat. Num. AAA8217487

• Gene Names: NOVA1; Nova-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NOVA1; NOVA1 siRNA (Human); RNA-binding protein Nova-1; Neuro-oncological ventral antigen 1; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; Ventral neuron-specific protein 1; NOVA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NOVA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 483

• Applicable Applications for NOVA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NOVA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NOVA1 sirna

siRNA to inhibit NOVA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 56549653
• NCBI GeneID: 4857
• NCBI Accession #: NP_002506.2
• NCBI GenBank Nucleotide #: NM_002515.2
• UniProt Accession #: P51513; Q6B004; A8K0S4; A8K4Q7; D3DS81; D3DS82
• Molecular Weight: 49,253 Da
• NCBI Official Full Name: RNA-binding protein Nova-1 isoform 1
• NCBI Official Synonym Full Names: neuro-oncological ventral antigen 1
• NCBI Official Symbol: NOVA1
• NCBI Official Synonym Symbols: Nova-1
• NCBI Protein Information: RNA-binding protein Nova-1
• UniProt Protein Name: RNA-binding protein Nova-1
• Protein Family: RNA-binding protein
• UniProt Gene Name: NOVA1
• UniProt Entry Name: NOVA1_HUMAN

NCBI Description

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

NOVA1: May regulate RNA splicing or metabolism in a specific subset of developing neurons. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 14q

Cellular Component: intracellular membrane-bound organelle; nucleolus; nucleus

Molecular Function: mRNA binding; RNA binding

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; synaptic transmission; RNA splicing; regulation of RNA metabolic process; locomotory behavior