NOTCH1 sirna

NOTCH1 siRNA (Human)

Cat. Num. AAA8234626

• Gene Names: NOTCH1; hN1; AOS5; TAN1; AOVD1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NOTCH1; NOTCH1 siRNA (Human); TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1; NOTCH1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NOTCH1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2555

• Applicable Applications for NOTCH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NOTCH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NOTCH1 sirna

siRNA to inhibit NOTCH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148833508
• NCBI GeneID: 4851
• NCBI Accession #: NP_060087.3
• NCBI GenBank Nucleotide #: NM_017617.3
• UniProt Accession #: P46531; Q59ED8; Q5SXM3
• Molecular Weight: 272,505 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 1 preproprotein
• NCBI Official Synonym Full Names: notch 1
• NCBI Official Symbol: NOTCH1
• NCBI Official Synonym Symbols: hN1; AOS5; TAN1; AOVD1
• NCBI Protein Information: neurogenic locus notch homolog protein 1
• UniProt Protein Name: Neurogenic locus notch homolog protein 1
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: NOTCH1
• UniProt Synonym Gene Names: TAN1; Notch 1; hN1; NEXT; NICD
• UniProt Entry Name: NOTC1_HUMAN

NCBI Description

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq, Jul 2008]

Uniprot Description

Notch 1: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation. In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Belongs to the NOTCH family.

Protein type: Transcription factor; Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral; Oncoprotein

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cell surface; acrosome; integral to membrane; plasma membrane; extracellular region; nucleus; cytosol; receptor complex

Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding; sequence-specific DNA binding; chromatin DNA binding; receptor activity; calcium ion binding; transcription factor activity

Biological Process: neural tube development; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; heart development; positive regulation of transcription, DNA-dependent; positive regulation of JAK-STAT cascade; response to lipopolysaccharide; response to corticosteroid stimulus; cell differentiation in spinal cord; negative regulation of BMP signaling pathway; compartment specification; positive regulation of endothelial cell differentiation; negative regulation of ossification; oligodendrocyte differentiation; somatic stem cell division; negative regulation of osteoblast differentiation; positive regulation of astrocyte differentiation; positive regulation of keratinocyte differentiation; positive regulation of cardiac muscle cell proliferation; negative regulation of photoreceptor cell differentiation; positive regulation of neuroblast proliferation; organ regeneration; Notch receptor processing; keratinocyte differentiation; branching morphogenesis of a tube; response to muramyl dipeptide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; determination of left/right symmetry; positive regulation of epithelial cell proliferation; foregut morphogenesis; endoderm development; cardiac muscle cell proliferation; cell fate specification; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of neurogenesis; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; astrocyte differentiation; tissue regeneration; cardiac muscle morphogensis; positive regulation of cell proliferation; forebrain development; heart looping; regulation of somitogenesis; positive regulation of BMP signaling pathway; mesenchymal cell development; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; hair follicle morphogenesis; in utero embryonic development; lumen formation; liver development; humoral immune response; activation of Notch receptor target transcription factor; negative regulation of oligodendrocyte differentiation; inflammatory response to antigenic stimulus; axonogenesis; negative regulation of catalytic activity; epithelial to mesenchymal transition; immune response; gene expression; spermatogenesis; sprouting angiogenesis; auditory receptor cell fate commitment; negative regulation of myoblast differentiation; positive regulation of cell migration; lung development

Disease: Adams-oliver Syndrome 5; Aortic Valve Disease 1