NEFL sirna

NEFL siRNA (Human)

Cat. Num. AAA8227864

• Gene Names: NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NEFL; NEFL siRNA (Human); NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein; NEFL sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NEFL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 543

• Applicable Applications for NEFL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NEFL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NEFL sirna

siRNA to inhibit NEFL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 105990539
• NCBI GeneID: 4747
• NCBI Accession #: NP_006149.2
• NCBI GenBank Nucleotide #: NM_006158.4
• UniProt Accession #: P07196; Q16154; Q8IU72; B9ZVN2
• Molecular Weight: 61,517 Da
• NCBI Official Full Name: neurofilament light polypeptide
• NCBI Official Synonym Full Names: neurofilament, light polypeptide
• NCBI Official Symbol: NEFL
• NCBI Official Synonym Symbols: NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
• NCBI Protein Information: neurofilament light polypeptide
• UniProt Protein Name: Neurofilament light polypeptide
• Protein Family: Neurofilament light polypeptide
• UniProt Gene Name: NEFL
• UniProt Synonym Gene Names: NF68; NFL; NF-L
• UniProt Entry Name: NFL_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Uniprot Description

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol

Molecular Function: protein binding, bridging; protein C-terminus binding; protein domain specific binding; identical protein binding; protein binding; phospholipase binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; retrograde axon cargo transport; axon regeneration in the peripheral nervous system; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; neuromuscular process controlling balance; locomotion; anterograde axon cargo transport; neurite morphogenesis; response to corticosterone stimulus

Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f