NDNL2 sirna

NDNL2 siRNA (Human)

Cat. Num. AAA8227197

• Gene Names: NDNL2; HCA4; NSE3; MAGEG1; MAGEL3; NSMCE3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NDNL2; NDNL2 siRNA (Human); HCA4; MAGEG1; Melanoma-associated antigen G1; Hepatocellular carcinoma-associated protein 4; MAGE-G1 antigen; Necdin-like protein 2; NDNL2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NDNL2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 304

• Applicable Applications for NDNL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NDNL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NDNL2 sirna

siRNA to inhibit NDNL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 20162572
• NCBI GeneID: 56160
• NCBI Accession #: NP_619649.1
• NCBI GenBank Nucleotide #: NM_138704.3
• UniProt Accession #: Q96MG7; Q8IW16; Q8TEI6; Q9H214
• Molecular Weight: 34,308 Da
• NCBI Official Full Name: melanoma-associated antigen G1
• NCBI Official Synonym Full Names: necdin-like 2, SMC5-SMC6 complex component
• NCBI Official Symbol: NDNL2
• NCBI Official Synonym Symbols: HCA4; NSE3; MAGEG1; MAGEL3; NSMCE3
• NCBI Protein Information: melanoma-associated antigen G1
• UniProt Protein Name: Melanoma-associated antigen G1
• UniProt Gene Name: NDNL2
• UniProt Synonym Gene Names: HCA4; MAGEG1
• UniProt Entry Name: MAGG1_HUMAN

NCBI Description

The protein encoded by this gene is part of the SMC5-6 chromatin reorganizing complex and is a member of the MAGE superfamily. This is an intronless gene. [provided by RefSeq, May 2011]

Uniprot Description

MAGE-G1: Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 15q13.1

Cellular Component: chromosome, telomeric region; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of protein ubiquitination; regulation of growth; DNA repair; DNA recombination