NDC80 sirna

NDC80 siRNA (Human)

Cat. Num. AAA8223076

• Gene Names: NDC80; HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NDC80; NDC80 siRNA (Human); HEC; HEC1; KNTC2; Kinetochore protein NDC80 homolog; Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC; NDC80 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NDC80 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 642

• Applicable Applications for NDC80 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NDC80 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NDC80 sirna

siRNA to inhibit NDC80 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5174457
• NCBI GeneID: 10403
• NCBI Accession #: NP_006092.1
• NCBI GenBank Nucleotide #: NM_006101.2
• UniProt Accession #: O14777; Q6PJX2
• Molecular Weight: 73,913 Da
• NCBI Official Full Name: kinetochore protein NDC80 homolog
• NCBI Official Synonym Full Names: NDC80 kinetochore complex component
• NCBI Official Symbol: NDC80
• NCBI Official Synonym Symbols: HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
• NCBI Protein Information: kinetochore protein NDC80 homolog
• UniProt Protein Name: Kinetochore protein NDC80 homolog
• Protein Family: Kinetochore protein
• UniProt Gene Name: NDC80
• UniProt Synonym Gene Names: HEC; HEC1; KNTC2; HsHec1
• UniProt Entry Name: NDC80_HUMAN

NCBI Description

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Uniprot Description

HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: kinetochore; membrane; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: mitosis; mitotic spindle organization and biogenesis; mitotic sister chromatid segregation; attachment of spindle microtubules to kinetochore during mitosis; establishment of mitotic spindle orientation; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; chromosome segregation