NCBP1 sirna

NCBP1 siRNA (Human)

Cat. Num. AAA8223546

• Gene Names: NCBP1; NCBP; Sto1; CBP80
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NCBP1; NCBP1 siRNA (Human); CBP80; NCBP; Nuclear cap-binding protein subunit 1; 80 kDa nuclear cap-binding protein; NCBP 80 kDa subunit; NCBP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NCBP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 790

• Applicable Applications for NCBP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NCBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NCBP1 sirna

siRNA to inhibit NCBP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4505343
• NCBI GeneID: 4686
• NCBI Accession #: NP_002477.1
• NCBI GenBank Nucleotide #: NM_002486.4
• UniProt Accession #: Q09161; Q59G76; Q5T1V0; Q5T7X2; B2R718
• Molecular Weight: 91,839 Da
• NCBI Official Full Name: nuclear cap-binding protein subunit 1
• NCBI Official Synonym Full Names: nuclear cap binding protein subunit 1, 80kDa
• NCBI Official Symbol: NCBP1
• NCBI Official Synonym Symbols: NCBP; Sto1; CBP80
• NCBI Protein Information: nuclear cap-binding protein subunit 1
• UniProt Protein Name: Nuclear cap-binding protein subunit 1
• UniProt Gene Name: NCBP1
• UniProt Synonym Gene Names: CBP80; NCBP; CBP80; NCBP 80 kDa subunit
• UniProt Entry Name: NCBP1_HUMAN

NCBI Description

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq, Jul 2008]

Uniprot Description

NCBP1: mediates U snRNA export from the nucleus. Binds to 5' capped mRNA. Found in aU snRNA export complex with RNUXA/PHAX, CBP20, CBP80, RAN, XPO1 and m7G-capped RNA. Interaction with RNUXA/PHAX. Heterodimer with NCBP2. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2, THOC4 and/or REFBP2.

Protein type: Translation; RNA processing; Spliceosome

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; mRNA cap complex; mitochondrion; cytoplasm; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: protein binding; RNA binding; RNA cap binding

Biological Process: transcription from RNA polymerase II promoter; spliceosomal snRNP biogenesis; viral reproduction; positive regulation of viral transcription; RNA splicing; positive regulation of mRNA 3'-end processing; histone mRNA metabolic process; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; mRNA capping; RNA elongation from RNA polymerase II promoter; mRNA catabolic process, nonsense-mediated decay; gene expression; RNA-mediated gene silencing; nuclear mRNA cis splicing, via U2-type spliceosome; mRNA 3'-end processing; termination of RNA polymerase II transcription; regulation of translational initiation; mRNA cleavage