MYO7A sirna

MYO7A siRNA (Human)

Cat. Num. AAA8216550

• Gene Names: MYO7A; DFNB2; MYU7A; NSRD2; USH1B; DFNA11; MYOVIIA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYO7A; MYO7A siRNA (Human); USH1B; Unconventional myosin-VIIa; MYO7A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MYO7A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2,166

• Applicable Applications for MYO7A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYO7A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYO7A sirna

siRNA to inhibit MYO7A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 189083798
• NCBI GeneID: 4647
• NCBI Accession #: NP_000251.3
• NCBI GenBank Nucleotide #: NM_000260.3
• UniProt Accession #: Q13402; P78427; Q13321; Q14785; Q92821; Q92822; B9A011; F8VUN5
• Molecular Weight: 249,165 Da
• NCBI Official Full Name: unconventional myosin-VIIa isoform 1
• NCBI Official Synonym Full Names: myosin VIIA
• NCBI Official Symbol: MYO7A
• NCBI Official Synonym Symbols: DFNB2; MYU7A; NSRD2; USH1B; DFNA11; MYOVIIA
• NCBI Protein Information: unconventional myosin-VIIa
• UniProt Protein Name: Unconventional myosin-VIIa
• Protein Family: Unconventional myosin
• UniProt Gene Name: MYO7A
• UniProt Synonym Gene Names: USH1B
• UniProt Entry Name: MYO7A_HUMAN

NCBI Description

This gene is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain, an actin-binding domain, a neck domain that interacts with other proteins, and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness, reduced vestibular function, and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

MYO7A: an actin-based motor molecule with ATPase activity and a calcium sensitive calmodulin binding subunit. May play a role in trafficking of ribbon- synaptic vesicle complexes and renewal of outer photoreceptor disks. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. Seven alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: stereocilium; photoreceptor outer segment; photoreceptor inner segment; lysosomal membrane; apical plasma membrane; cytoplasm; melanosome; synapse; cell cortex; cytosol; photoreceptor connecting cilium

Molecular Function: calmodulin binding; actin filament binding; microfilament motor activity; protein domain specific binding; protein binding; protein homodimerization activity; spectrin binding; actin-dependent ATPase activity; ADP binding; ATP binding

Biological Process: actin filament-based movement; metabolic process; phagolysosome formation; intracellular protein transport; eye photoreceptor cell development; pigment granule transport; sensory perception of sound; visual perception; lysosome organization and biogenesis; sensory perception of light stimulus; auditory receptor cell stereocilium organization and biogenesis; equilibrioception; post-embryonic organ morphogenesis

Disease: Deafness, Autosomal Dominant 11; Deafness, Autosomal Recessive 2; Usher Syndrome, Type I