MYO1G sirna

MYO1G siRNA (Human)

Cat. Num. AAA8234182

• Gene Names: MYO1G; HA2; MHAG; HLA-HA2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYO1G; MYO1G siRNA (Human); HA2; Unconventional myosin-Ig; MYO1G sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MYO1G siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1018

• Applicable Applications for MYO1G sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYO1G gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYO1G sirna

siRNA to inhibit MYO1G expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 239582755
• NCBI GeneID: 64005
• NCBI Accession #: NP_149043.2
• NCBI GenBank Nucleotide #: NM_033054.2
• UniProt Accession #: B0I1T2; Q8TEI9; Q8TES2; Q96BE2; Q96RI5; Q96RI6
• Molecular Weight: 82,279 Da
• NCBI Official Full Name: unconventional myosin-Ig
• NCBI Official Synonym Full Names: myosin IG
• NCBI Official Symbol: MYO1G
• NCBI Official Synonym Symbols: HA2; MHAG; HLA-HA2
• NCBI Protein Information: unconventional myosin-Ig
• UniProt Protein Name: Unconventional myosin-Ig
• Protein Family: Unconventional myosin
• UniProt Gene Name: MYO1G
• UniProt Synonym Gene Names: HA2; mHag HA-2
• UniProt Entry Name: MYO1G_HUMAN

NCBI Description

MYO1G is a plasma membrane-associated class I myosin (see MIM 601478) that is abundant in T and B lymphocytes and mast cells (Pierce et al., 2001 [PubMed 11544309]; Patino-Lopez et al., 2010 [PubMed 20071333]).[supplied by OMIM, Jun 2010]

Uniprot Description

MYO1G: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Contractile; Motor

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: microvillus; membrane; lamellipodium; plasma membrane; myosin complex; phagocytic cup; filopodium

Molecular Function: calmodulin binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; motor activity; phosphatidylinositol-3,4-bisphosphate binding; actin binding; ATP binding

Biological Process: exocytosis; metabolic process; T cell mediated immunity; cell-substrate adhesion