MYBPC1 sirna

MYBPC1 siRNA (Human)

Cat. Num. AAA8213156

• Gene Names: MYBPC1; LCCS4; MYBPCC; MYBPCS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYBPC1; MYBPC1 siRNA (Human); MYBPCS; Myosin-binding protein C. slow-type; Slow MyBP-C; C-protein. skeletal muscle slow isoform; MYBPC1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MYBPC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1173

• Applicable Applications for MYBPC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYBPC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYBPC1 sirna

siRNA to inhibit MYBPC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 360039217
• NCBI GeneID: 4604
• NCBI Accession #: NP_001241647.1
• NCBI GenBank Nucleotide #: NM_001254718.1
• UniProt Accession #: Q00872; Q15497; Q17RR7; Q569K7; B4DKR5; B7Z8G8; B7ZL02; B7ZL09; B7ZL10; E7ESM5; E7EWS6; G3XAE8
• Molecular Weight: 129,074 Da
• NCBI Official Full Name: myosin-binding protein C, slow-type isoform 5
• NCBI Official Synonym Full Names: myosin binding protein C, slow type
• NCBI Official Symbol: MYBPC1
• NCBI Official Synonym Symbols: LCCS4; MYBPCC; MYBPCS
• NCBI Protein Information: myosin-binding protein C, slow-type
• UniProt Protein Name: Myosin-binding protein C, slow-type
• Protein Family: Myosin-binding protein
• UniProt Gene Name: MYBPC1
• UniProt Synonym Gene Names: MYBPCS; Slow MyBP-C
• UniProt Entry Name: MYPC1_HUMAN

NCBI Description

This gene encodes a member of the myosin-binding protein C family. Myosin-binding protein C family members are myosin-associated proteins found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The encoded protein is the slow skeletal muscle isoform of myosin-binding protein C and plays an important role in muscle contraction by recruiting muscle-type creatine kinase to myosin filaments. Mutations in this gene are associated with distal arthrogryposis type I. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

MYBPC1: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC1 are the cause of arthrogryposis, distal, type 1B (DA1B). A form of distal arthrogryposis, a disease characterized by congenital joint contractures that mainly involve two or more distal parts of the limbs, in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Defects in MYBPC1 may be a cause of autosomal recessive lethal congenital contractural syndrome (LCCS), a severe, neonatally lethal form of arthrogryposis. Belongs to the immunoglobulin superfamily. MyBP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Myosin-binding; Actin-binding

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: myofibril; cytosol

Molecular Function: protein binding; structural constituent of muscle; titin binding; actin binding

Biological Process: cell adhesion; muscle filament sliding

Disease: Lethal Congenital Contracture Syndrome 4; Arthrogryposis, Distal, Type 1b