MTCH1 sirna

MTCH1 siRNA (Human)

Cat. Num. AAA8239764

• Gene Names: MTCH1; PSAP; PIG60; CGI-64; SLC25A49
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MTCH1; MTCH1 siRNA (Human); PSAP; Mitochondrial carrier homolog 1; Presenilin-associated protein; MTCH1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MTCH1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 389

• Applicable Applications for MTCH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MTCH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MTCH1 sirna

siRNA to inhibit MTCH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 410442521
• NCBI GeneID: 23787
• NCBI Accession #: NP_001258570.1
• NCBI GenBank Nucleotide #: NM_001271641.1
• UniProt Accession #: Q9NZJ7; Q6PK60; Q6UX45; Q7L465; Q9BW23; Q9NZR6; Q9UJZ5; A8KAX5; B2RCE3
• Molecular Weight: 19,058 Da
• NCBI Official Full Name: mitochondrial carrier homolog 1 PSAP-LL
• NCBI Official Synonym Full Names: mitochondrial carrier 1
• NCBI Official Symbol: MTCH1
• NCBI Official Synonym Symbols: PSAP; PIG60; CGI-64; SLC25A49
• NCBI Protein Information: mitochondrial carrier homolog 1
• UniProt Protein Name: Mitochondrial carrier homolog 1
• Protein Family: Mitochondrial carrier
• UniProt Gene Name: MTCH1
• UniProt Synonym Gene Names: PSAP
• UniProt Entry Name: MTCH1_HUMAN

NCBI Description

This gene encodes a member of the mitochondrial carrier family. The encoded protein is localized to the mitochondrion inner membrane and induces apoptosis independent of the proapoptotic proteins Bax and Bak. Pseudogenes on chromosomes 6 and 11 have been identified for this gene. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Oct 2012]

Uniprot Description

MTCH1: Potential mitochondrial transporter. May play a role in apoptosis. Belongs to the mitochondrial carrier family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane; intracellular

Molecular Function: protein binding

Biological Process: caspase activation; transport; positive regulation of apoptosis; regulation of signal transduction; neuronal ion channel clustering