MTA1 sirna

MTA1 siRNA (Human)

Cat. Num. AAA8240049

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MTA1; MTA1 siRNA (Human); Metastasis-associated protein MTA1; MTA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MTA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 430

• Applicable Applications for MTA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MTA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MTA1 sirna

siRNA to inhibit MTA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 322506112
• NCBI GeneID: 9112
• NCBI Accession #: NP_001190187.1
• NCBI GenBank Nucleotide #: NM_001203258.1
• UniProt Accession #: Q13330; Q86SW2; Q8NFI8; Q96GI8; A5PLK4
• Molecular Weight: 79,192 Da
• NCBI Official Full Name: metastasis-associated protein MTA1 isoform MTA1s
• NCBI Official Synonym Full Names: metastasis associated 1
• NCBI Official Symbol: MTA1
• NCBI Protein Information: metastasis-associated protein MTA1
• UniProt Protein Name: Metastasis-associated protein MTA1
• Protein Family: Mating type protein
• UniProt Gene Name: MTA1
• UniProt Entry Name: MTA1_HUMAN

NCBI Description

This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

MTA1: metastasis-associated protein. A component of the NuRD ATP-dependent chromatin remodeling and histone deacetylase complex. Interacts with HDAC1. Expressed at high levels in metastatic cells. Two alternatively spliced isoforms have been described. The long isoform is a co-repressor of estrogen receptor (ER). The short isoform binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 14q32.3

Cellular Component: nucleoplasm; microtubule; intracellular membrane-bound organelle; cytoplasm; nuclear envelope; NuRD complex; nucleus

Molecular Function: protein binding; zinc ion binding; transcription coactivator activity; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: locomotor rhythm; proteasomal ubiquitin-dependent protein catabolic process; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; double-strand break repair; regulation of inflammatory response; response to lipopolysaccharide; response to ionizing radiation; circadian regulation of gene expression; signal transduction; regulation of gene expression, epigenetic; entrainment of circadian clock by photoperiod