MSMB sirna

MSMB siRNA (Human)

Cat. Num. AAA8201343

• Gene Names: MSMB; MSP; PSP; IGBF; MSPB; PN44; PRPS; HPC13; PSP57; PSP94; PSP-94
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MSMB; MSMB siRNA (Human); PRSP; Beta-microseminoprotein; Immunoglobulin-binding factor; IGBF; PN44; Prostate secreted seminal plasma protein; Prostate secretory protein of 94 amino acids; PSP-94; PSP94; Seminal plasma beta-inhibin; MSMB sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MSMB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 114

• Applicable Applications for MSMB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MSMB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MSMB sirna

siRNA to inhibit MSMB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4557036
• NCBI GeneID: 4477
• NCBI Accession #: NP_002434.1
• NCBI GenBank Nucleotide #: NM_002443.3
• UniProt Accession #: P08118; P11999; Q13125; Q6IAY9; Q9UC59; B1API6
• Molecular Weight: 8,778 Da
• NCBI Official Full Name: beta-microseminoprotein isoform a
• NCBI Official Synonym Full Names: microseminoprotein, beta-
• NCBI Official Symbol: MSMB
• NCBI Official Synonym Symbols: MSP; PSP; IGBF; MSPB; PN44; PRPS; HPC13; PSP57; PSP94; PSP-94
• NCBI Protein Information: beta-microseminoprotein
• UniProt Protein Name: Beta-microseminoprotein
• Protein Family: Methanesulfonate monooxygenase hydroxylase
• UniProt Gene Name: MSMB
• UniProt Synonym Gene Names: PRSP; IGBF; PSP-94; PSP94
• UniProt Entry Name: MSMB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer. Two alternatively spliced transcript variants encoding different isoforms are described for this gene. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MSMB: Defects in MSMB are the cause of susceptibility to prostate cancer hereditary type 13 (HPC13). It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Belongs to the beta-microseminoprotein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; nucleus

Disease: Prostate Cancer, Hereditary, 13