MSH6 sirna

MSH6 siRNA (Human)

Cat. Num. AAA8234027

• Gene Names: MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MSH6; MSH6 siRNA (Human); GTBP; DNA mismatch repair protein Msh6; hMSH6; G/T mismatch-binding protein; GTMBP; MutS-alpha 160 kDa subunit; p160; MSH6 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MSH6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1360

• Applicable Applications for MSH6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MSH6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MSH6 sirna

siRNA to inhibit MSH6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4504191
• NCBI GeneID: 2956
• NCBI Accession #: NP_000170.1
• NCBI GenBank Nucleotide #: NM_000179.2
• UniProt Accession #: P52701; O43706; O43917; Q8TCX4; Q9BTB5; B4DF41; B4E3I4; F5H2F9
• Molecular Weight: 119,796 Da
• NCBI Official Full Name: DNA mismatch repair protein Msh6 isoform 1
• NCBI Official Synonym Full Names: mutS homolog 6
• NCBI Official Symbol: MSH6
• NCBI Official Synonym Symbols: GTBP; HSAP; p160; GTMBP; HNPCC5
• NCBI Protein Information: DNA mismatch repair protein Msh6
• UniProt Protein Name: DNA mismatch repair protein Msh6
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: MSH6
• UniProt Synonym Gene Names: GTBP; hMSH6; GTBP; GTMBP; p160
• UniProt Entry Name: MSH6_HUMAN

NCBI Description

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]

Uniprot Description

MSH6: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Belongs to the DNA mismatch repair MutS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p16

Cellular Component: nucleoplasm; nuclear chromosome; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; nuclear chromatin; plasma membrane; MutSalpha complex

Molecular Function: DNA-dependent ATPase activity; protein homodimerization activity; single thymine insertion binding; ATPase activity; oxidized purine DNA binding; magnesium ion binding; ADP binding; methylated histone residue binding; mismatched DNA binding; protein binding; four-way junction DNA binding; single guanine insertion binding; double-stranded DNA binding; guanine/thymine mispair binding; MutLalpha complex binding; chromatin binding; ATP binding

Biological Process: positive regulation of helicase activity; negative regulation of DNA recombination; mismatch repair; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; somatic hypermutation of immunoglobulin genes; isotype switching; somatic recombination of immunoglobulin gene segments; determination of adult life span; DNA repair; maintenance of DNA repeat elements; meiotic mismatch repair; response to UV

Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 5; Endometrial Cancer; Mismatch Repair Cancer Syndrome