MOCS3 sirna

MOCS3 siRNA (Human)

Cat. Num. AAA8217448

• Gene Names: MOCS3; UBA4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MOCS3; MOCS3 siRNA (Human); UBA4; Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; MOCS3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MOCS3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 460

• Applicable Applications for MOCS3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MOCS3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MOCS3 sirna

siRNA to inhibit MOCS3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7657339
• NCBI GeneID: 27304
• NCBI Accession #: NP_055299.1
• NCBI GenBank Nucleotide #: NM_014484.4
• UniProt Accession #: O95396
• Molecular Weight: 49,669 Da
• NCBI Official Full Name: adenylyltransferase and sulfurtransferase MOCS3
• NCBI Official Synonym Full Names: molybdenum cofactor synthesis 3
• NCBI Official Symbol: MOCS3
• NCBI Official Synonym Symbols: UBA4
• NCBI Protein Information: adenylyltransferase and sulfurtransferase MOCS3
• UniProt Protein Name: Adenylyltransferase and sulfurtransferase MOCS3
• UniProt Gene Name: MOCS3
• UniProt Synonym Gene Names: MPT synthase sulfurylase
• UniProt Entry Name: MOCS3_HUMAN

NCBI Description

Molybdenum cofactor (MoCo) is necessary for the function of all molybdoenzymes. The protein encoded by this gene adenylates and activates molybdopterin synthase, an enzyme required for biosynthesis of MoCo. This gene contains no introns. A pseudogene of this gene is present on chromosome 14. [provided by RefSeq, Nov 2012]

Uniprot Description

MOCS3: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

Protein type: EC 2.8.1.11; Transferase; Ligase; EC 2.7.7.80

Chromosomal Location of Human Ortholog: 20q13.13

Cellular Component: cytosol

Molecular Function: sulfurtransferase activity; protein binding; thiosulfate sulfurtransferase activity; metal ion binding; URM1 activating enzyme activity; ATP binding; nucleotidyltransferase activity

Biological Process: tRNA wobble uridine modification; protein urmylation; vitamin metabolic process; enzyme active site formation via L-cysteine persulfide; protein amino acid adenylylation; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process