MKRN1 sirna

MKRN1 siRNA (Human)

Cat. Num. AAA8241087

• Gene Names: MKRN1; RNF61
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MKRN1; MKRN1 siRNA (Human); RNF61; E3 ubiquitin-protein ligase makorin-1; RING finger protein 61; MKRN1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MKRN1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 329

• Applicable Applications for MKRN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MKRN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MKRN1 sirna

siRNA to inhibit MKRN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 223468622
• NCBI GeneID: 23608
• NCBI Accession #: NP_001138597.1
• NCBI GenBank Nucleotide #: NM_001145125.1
• UniProt Accession #: Q9UHC7; Q256Y7; Q59G11; Q6GSF1; Q9H0G0; Q9UEZ7; Q9UHW2; A4D1T7; B3KXB4
• Molecular Weight: 35,224 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase makorin-1 isoform 2
• NCBI Official Synonym Full Names: makorin ring finger protein 1
• NCBI Official Symbol: MKRN1
• NCBI Official Synonym Symbols: RNF61
• NCBI Protein Information: E3 ubiquitin-protein ligase makorin-1
• UniProt Protein Name: E3 ubiquitin-protein ligase makorin-1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: MKRN1
• UniProt Synonym Gene Names: RNF61
• UniProt Entry Name: MKRN1_HUMAN

NCBI Description

This gene encodes a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription. Substrates for this protein's E3 ubiquitin ligase activity include the capsid protein of the West Nile virus and the catalytic subunit of the telomerase ribonucleoprotein. This protein controls cell cycle arrest and apoptosis by regulating p21, a cell cycle regulator, and the tumor suppressor protein p53. Pseudogenes of this gene are present on chromosomes 1, 3, 9, 12 and 20, and on the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

MKRN1: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 7q34

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; chromatin binding; ligase activity

Biological Process: protein polyubiquitination