MICAL3 sirna

MICAL3 siRNA (Human)

Cat. Num. AAA8222967

• Gene Names: MICAL3; MICAL-3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MICAL3; MICAL3 siRNA (Human); KIAA0819; KIAA1364; Protein-methionine sulfoxide oxidase MICAL3; Molecule interacting with CasL protein 3; MICAL-3; MICAL3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MICAL3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 966

• Applicable Applications for MICAL3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MICAL3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MICAL3 sirna

siRNA to inhibit MICAL3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 170172518
• NCBI GeneID: 57553
• NCBI Accession #: NP_001116203.1
• NCBI GenBank Nucleotide #: NM_001122731.2
• UniProt Accession #: Q7RTP6; O94909; Q5U4P4; Q6ICK4; Q96DF2; Q9P2I3; B2RXJ5; E9PEF0
• Molecular Weight: 121,173 Da
• NCBI Official Full Name: protein-methionine sulfoxide oxidase MICAL3 isoform 3
• NCBI Official Synonym Full Names: microtubule associated monooxygenase, calponin and LIM domain containing 3
• NCBI Official Symbol: MICAL3
• NCBI Official Synonym Symbols: MICAL-3
• NCBI Protein Information: protein-methionine sulfoxide oxidase MICAL3
• UniProt Protein Name: Protein-methionine sulfoxide oxidase MICAL3
• Protein Family: Protein-methionine sulfoxide oxidase
• UniProt Gene Name: MICAL3
• UniProt Synonym Gene Names: KIAA0819; KIAA1364; MICAL-3
• UniProt Entry Name: MICA3_HUMAN

Uniprot Description

MICAL3: a monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process. Interacts with RAB1B. Interacts with ERC1 and RAB8A. Five human isoforms are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.13.-

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; plasma membrane; nucleus

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; zinc ion binding; actin binding; Rab GTPase binding

Biological Process: actin filament depolymerization; exocytosis; cytoskeleton organization and biogenesis