MED7 sirna

MED7 siRNA (Human)

Cat. Num. AAA829725

• Gene Names: MED7; ARC34; CRSP9; CRSP33
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MED7; MED7 siRNA (Human); ARC34; CRSP9; Mediator of RNA polymerase II transcription subunit 7; hMED7; Activator-recruited cofactor 34 kDa component; Cofactor required for Sp1 transcriptional activation subunit 9; CRSP complex subunit 9; Mediator complex subunit 7; RNA polymerase transcriptional regulation mediator subunit 7 homolog; Transcriptional coactivator CRSP33; MED7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MED7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 233

• Applicable Applications for MED7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MED7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MED7 sirna

siRNA to inhibit MED7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 155030188
• NCBI GeneID: 9443
• NCBI Accession #: NP_001094286.1
• NCBI GenBank Nucleotide #: NM_001100816.1
• UniProt Accession #: O43513
• Molecular Weight: 27,245 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 7
• NCBI Official Synonym Full Names: mediator complex subunit 7
• NCBI Official Symbol: MED7
• NCBI Official Synonym Symbols: ARC34; CRSP9; CRSP33
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 7
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 7
• Protein Family: Putative mediator of RNA polymerase II transcription
• UniProt Gene Name: MED7
• UniProt Synonym Gene Names: ARC34; CRSP9; hMED7; ARC34; CRSP complex subunit 9
• UniProt Entry Name: MED7_HUMAN

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MED7: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 7 family.

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: nucleoplasm; transcription factor complex; nuclear membrane; mitochondrion; Srb-mediator complex; nucleus

Molecular Function: protein binding; transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; stem cell maintenance; gene expression