MC1R sirna

MC1R siRNA (Human)

Cat. Num. AAA8238951

• Gene Names: MC1R; CMM5; MSH-R; SHEP2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MC1R; MC1R siRNA (Human); MSHR; Melanocyte-stimulating hormone receptor; MSH-R; Melanocortin receptor 1; MC1-R; MC1R sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MC1R siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 317

• Applicable Applications for MC1R sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MC1R gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MC1R sirna

siRNA to inhibit MC1R expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 193083134
• NCBI GeneID: 4157
• NCBI Accession #: NP_002377.4
• NCBI GenBank Nucleotide #: NM_002386.3
• UniProt Accession #: Q01726; Q66K38; Q6UR93; Q8WWX6; Q8WWX7; Q96I33; Q96RU4; Q9UBF7; Q9UN58; Q9UN59; Q9UN60; Q9UN61
• Molecular Weight: 34,706 Da
• NCBI Official Full Name: melanocyte-stimulating hormone receptor
• NCBI Official Synonym Full Names: melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
• NCBI Official Symbol: MC1R
• NCBI Official Synonym Symbols: CMM5; MSH-R; SHEP2
• NCBI Protein Information: melanocyte-stimulating hormone receptor
• UniProt Protein Name: Melanocyte-stimulating hormone receptor
• Protein Family: Melanocyte-stimulating hormone receptor
• UniProt Gene Name: MC1R
• UniProt Synonym Gene Names: MSHR; MSH-R; MC1-R
• UniProt Entry Name: MSHR_HUMAN

NCBI Description

This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq, Jul 2008]

Uniprot Description

MC1R: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5). Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; hormone binding; melanocortin receptor activity; ubiquitin protein ligase binding; melanocyte stimulating hormone receptor activity; peptide receptor activity, G-protein coupled

Biological Process: melanin biosynthetic process; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of protein kinase B signaling cascade; pigmentation; UV protection; multicellular organismal development; positive regulation of cAMP biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; sensory perception of pain

Disease: Albinism, Oculocutaneous, Type Ii; Increased Analgesia From Kappa-opioid Receptor Agonist, Female-specific; Melanoma, Cutaneous Malignant, Susceptibility To, 5; Skin/hair/eye Pigmentation, Variation In, 2