MARK3 sirna

MARK3 siRNA (Human)

Cat. Num. AAA8216595

• Gene Names: MARK3; KP78; CTAK1; PAR1A; Par-1a
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MARK3; MARK3 siRNA (Human); CTAK1; EMK2; MAP/microtubule affinity-regulating kinase 3; C-TAK1; cTAK1; Cdc25C-associated protein kinase 1; ELKL motif kinase 2; EMK-2; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Par-1a; Serine/threonine-protein kinase p78; MARK3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MARK3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 659

• Applicable Applications for MARK3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MARK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MARK3 sirna

siRNA to inhibit MARK3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 731441413
• NCBI GeneID: 4140
• NCBI Accession #: NP_001122390.2
• NCBI GenBank Nucleotide #: NM_001128918.2
• UniProt Accession #: P27448; O60219; Q86TT8; Q8TB41; Q8WX83; Q96RG1; Q9UMY9; Q9UN34
• Molecular Weight: 74,151 Da
• NCBI Official Full Name: MAP/microtubule affinity-regulating kinase 3 isoform a
• NCBI Official Synonym Full Names: MAP/microtubule affinity-regulating kinase 3
• NCBI Official Symbol: MARK3
• NCBI Official Synonym Symbols: KP78; CTAK1; PAR1A; Par-1a
• NCBI Protein Information: MAP/microtubule affinity-regulating kinase 3
• UniProt Protein Name: MAP/microtubule affinity-regulating kinase 3
• Protein Family: MAP/microtubule affinity-regulating kinase
• UniProt Gene Name: MARK3
• UniProt Synonym Gene Names: CTAK1; EMK2; cTAK1; EMK-2; Par-1a
• UniProt Entry Name: MARK3_HUMAN

NCBI Description

The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

MARK3: an ubiquitous serine/threonine kinase of the CAMKL family. Phosphorylates Cdc25C on serine 216 and promotes 14-3-3 protein binding. A positive regulator of Wnt signalling. Ubiquitously expressed in human tissues and cell lines. Seven alternatively spliced isoforms have been reported.

Protein type: Protein kinase, CAMK; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMKL family; MARK subfamily

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: protein amino acid phosphorylation