MAPKAPK3 sirna

MAPKAPK3 siRNA (Human)

Cat. Num. AAA8201683

• Gene Names: MAPKAPK3; 3PK; MK-3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAPKAPK3; MAPKAPK3 siRNA (Human); MAP kinase-activated protein kinase 3; MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP-K3; MAPKAPK-3; MK-3; Chromosome 3p kinase; 3pK; MAPKAPK3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MAPKAPK3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 382

• Applicable Applications for MAPKAPK3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MAPKAPK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAPKAPK3 sirna

siRNA to inhibit MAPKAPK3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 345441756
• NCBI GeneID: 7867
• NCBI Accession #: NP_001230854.1
• NCBI GenBank Nucleotide #: NM_001243925.1
• UniProt Accession #: Q16644; B5BU67
• Molecular Weight: 42,987 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 3
• NCBI Official Symbol: MAPKAPK3
• NCBI Official Synonym Symbols: 3PK; MK-3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• NCBI Protein Information: MAP kinase-activated protein kinase 3
• UniProt Protein Name: MAP kinase-activated protein kinase 3
• Protein Family: MAP kinase-activated protein kinase
• UniProt Gene Name: MAPKAPK3
• UniProt Synonym Gene Names: MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP-K3; MAPKAPK-3; MK-3; 3pK
• UniProt Entry Name: MAPK3_HUMAN

NCBI Description

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

MAPKAPK3: a member of the MAPKAPK family of protein kinases. Activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.1; Kinase, protein; CAMK group; MAPKAPK family; MAPKAPK subfamily

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; cytosol; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; peptidyl-serine phosphorylation; response to cytokine stimulus; Ras protein signal transduction; toll-like receptor signaling pathway; response to stress; innate immune response; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway; toll-like receptor 4 signaling pathway