LMBR1 sirna

LMBR1 siRNA (Human)

Cat. Num. AAA8213248

• Gene Names: LMBR1; LSS; TPT; ZRS; ACHP; PPD2; THYP; DIF14; C7orf2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LMBR1; LMBR1 siRNA (Human); C7orf2; DIF14; Limb region 1 protein homolog; Differentiation-related gene 14 protein; LMBR1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LMBR1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 490

• Applicable Applications for LMBR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LMBR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LMBR1 sirna

siRNA to inhibit LMBR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21362112
• NCBI GeneID: 64327
• NCBI Accession #: NP_071903.2
• NCBI GenBank Nucleotide #: NM_022458.3
• UniProt Accession #: Q8WVP7; Q8N3E3; Q96QZ5; Q9H5N0; Q9HAG9; Q9UDN5; Q9Y6U2; A4D242
• Molecular Weight: 59,458 Da
• NCBI Official Full Name: limb region 1 protein homolog
• NCBI Official Synonym Full Names: limb development membrane protein 1
• NCBI Official Symbol: LMBR1
• NCBI Official Synonym Symbols: LSS; TPT; ZRS; ACHP; PPD2; THYP; DIF14; C7orf2
• NCBI Protein Information: limb region 1 protein homolog
• UniProt Protein Name: Limb region 1 protein homolog
• Protein Family: Limb region 1 protein
• UniProt Gene Name: LMBR1
• UniProt Synonym Gene Names: C7orf2; DIF14
• UniProt Entry Name: LMBR1_HUMAN

NCBI Description

This gene encodes a member of the LMBR1-like membrane protein family. Another member of this protein family has been shown to be a lipocalin transmembrane receptor. A highly conserved, cis-acting regulatory module for the sonic hedgehog gene is located within an intron of this gene. Consequently, disruption of this genic region can alter sonic hedgehog expression and affect limb patterning, but it is not known if this gene functions directly in limb development. Mutations and chromosomal deletions and rearrangements in this genic region are associated with acheiropody and preaxial polydactyly, which likely result from altered sonic hedgehog expression. [provided by RefSeq, Jul 2008]

Uniprot Description

LMBR1: Putative membrane receptor. Defects in LMBR1 are associated with preaxial polydactyly type 2 (PPD2); also known as polydactyly of triphalangeal thumb. Polydactyly consists of duplication of the distal phalanx. The thumb in PPD2 is usually opposable and possesses a normal metacarpal. The mutations do not change the normal expression of LMBR1, but alter the expression of SHH by disrupting a long-range, cis-regulatory element of that gene. Defects in LMBR1 are the cause of acheiropody (ACHP). Acheiropody is a very rare condition characterized by bilateral congenital amputations of the hands and feet. The specific malformative phenotype consists of a complete amputation of the distal epiphysis of the humerus, amputation of the tibial diaphysis, and aplasia of the radius, ulna, fibula, and of all the bones of the hands and feet. This syndrome of autosomal recessive inheritance has only been observed in Brazil so far. Defects in LMBR1 are a cause of syndactyly type 4 (SDTY4). SDTY4 is a very rare congenital distal limb malformation characterized by complete bilateral syndactyly (involving all digits 1 to 5). A frequent association with polydactyly (with six metacarpals and six digits) has been reported. Feet are affected occasionally. The condition is inherited as an autosomal dominant trait. Belongs to the LIMR family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: integral to membrane

Biological Process: embryonic digit morphogenesis

Disease: Syndactyly, Type Iv; Polydactyly, Preaxial Ii; Laurin-sandrow Syndrome; Tibia, Hypoplasia Or Aplasia Of, With Polydactyly; Acheiropody