LITAF sirna

LITAF siRNA (Human)

Cat. Num. AAA8208496

• Gene Names: LITAF; PIG7; SIMPLE; TP53I7
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LITAF; LITAF siRNA (Human); PIG7; SIMPLE; Lipopolysaccharide-induced tumor necrosis factor-alpha factor; LPS-induced TNF-alpha factor; Small integral membrane protein of lysosome/late endosome; p53-induced gene 7 protein; LITAF sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LITAF siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 161

• Applicable Applications for LITAF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LITAF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LITAF sirna

siRNA to inhibit LITAF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 210147505
• NCBI GeneID: 9516
• NCBI Accession #: NP_001129944.1
• NCBI GenBank Nucleotide #: NM_001136472.1
• UniProt Accession #: Q99732; Q05DW0; Q9C0L6; D3DUG1; G5E9K0
• Molecular Weight: 15,824 Da
• NCBI Official Full Name: lipopolysaccharide-induced tumor necrosis factor-alpha factor isoform 1
• NCBI Official Synonym Full Names: lipopolysaccharide-induced TNF factor
• NCBI Official Symbol: LITAF
• NCBI Official Synonym Symbols: PIG7; SIMPLE; TP53I7
• NCBI Protein Information: lipopolysaccharide-induced tumor necrosis factor-alpha factor
• UniProt Protein Name: Lipopolysaccharide-induced tumor necrosis factor-alpha factor
• Protein Family: Lipopolysaccharide-induced tumor necrosis factor-alpha factor
• UniProt Gene Name: LITAF
• UniProt Synonym Gene Names: PIG7; SIMPLE; LPS-induced TNF-alpha factor
• UniProt Entry Name: LITAF_HUMAN

NCBI Description

Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

LITAF: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF- alpha) gene expression. Interacts with NEDD4. Interacts with WWOX. Isoform 2 may interact with STAT6. By bacterial lipopolysaccharides (LPS) or p53/TP53. In monocytes by the Bacillus Calmette-Guerin (BCG). Ubiquitously and abundantly expressed. Expressed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 16p13.13

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; lysosomal membrane; cytoplasm; plasma membrane

Molecular Function: protein binding; signal transducer activity; WW domain binding

Biological Process: regulation of transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; apoptosis; negative regulation of NF-kappaB import into nucleus; signal transduction; aging; regulation of cytokine production

Disease: Charcot-marie-tooth Disease, Demyelinating, Type 1c