LEPREL4 sirna

LEPREL4 siRNA (Human)

Cat. Num. AAA8238527

• Gene Names: P3H4; NO55; SC65; NOL55; LEPREL4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LEPREL4; LEPREL4 siRNA (Human); NOL55; SC65; Synaptonemal complex protein SC65; Leprecan-like protein 4; Nucleolar autoantigen No55; LEPREL4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LEPREL4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 437

• Applicable Applications for LEPREL4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LEPREL4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LEPREL4 sirna

siRNA to inhibit LEPREL4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5454038
• NCBI GeneID: 10609
• NCBI Accession #: NP_006446.1
• NCBI GenBank Nucleotide #: NM_006455.2
• UniProt Accession #: Q92791; Q53GI6; Q9H4F6
• Molecular Weight: 50,381 Da
• NCBI Official Full Name: synaptonemal complex protein SC65
• NCBI Official Synonym Full Names: prolyl 3-hydroxylase family member 4 (non-enzymatic)
• NCBI Official Symbol: P3H4
• NCBI Official Synonym Symbols: NO55; SC65; NOL55; LEPREL4
• NCBI Protein Information: synaptonemal complex protein SC65
• UniProt Protein Name: Synaptonemal complex protein SC65
• UniProt Gene Name: LEPREL4
• UniProt Synonym Gene Names: NOL55; SC65
• UniProt Entry Name: SC65_HUMAN

NCBI Description

This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein, with a predicted molecular weight of 50 kDa, appears to be localized in the particulate compartment of the interphase nucleolus, with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes. [provided by RefSeq, Jul 2008]

Uniprot Description

SC65: This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein, with a predicted molecular weight of 50 kDa, appears to be localized in the particulate compartment of the interphase nucleolus, with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes. [provided by RefSeq, Jul 2008]

Protein type: Nucleolus; Unknown function

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: condensed nuclear chromosome; nucleolus; synaptonemal complex

Biological Process: synaptonemal complex assembly