LCLAT1 sirna

LCLAT1 siRNA (Human)

Cat. Num. AAA8240244

• Gene Names: LCLAT1; LYCAT; AGPAT8; ALCAT1; 1AGPAT8; UNQ1849; HSRG1849
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LCLAT1; LCLAT1 siRNA (Human); AGPAT8; ALCAT1; LYCAT; Lysocardiolipin acyltransferase 1; 1-acylglycerol-3-phosphate O-acyltransferase 8; 1-AGP acyltransferase 8; 1-AGPAT 8; Acyl-CoA:lysocardiolipin acyltransferase 1; LCLAT1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LCLAT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 376

• Applicable Applications for LCLAT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LCLAT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LCLAT1 sirna

siRNA to inhibit LCLAT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 50659059
• NCBI GeneID: 253558
• NCBI Accession #: NP_001002257.1
• NCBI GenBank Nucleotide #: NM_001002257.2
• UniProt Accession #: Q6UWP7; Q8N1Q7; A6H8Z7
• Molecular Weight: 44,561 Da
• NCBI Official Full Name: lysocardiolipin acyltransferase 1 isoform 2
• NCBI Official Synonym Full Names: lysocardiolipin acyltransferase 1
• NCBI Official Symbol: LCLAT1
• NCBI Official Synonym Symbols: LYCAT; AGPAT8; ALCAT1; 1AGPAT8; UNQ1849; HSRG1849
• NCBI Protein Information: lysocardiolipin acyltransferase 1
• UniProt Protein Name: Lysocardiolipin acyltransferase 1
• Protein Family: Lysocardiolipin acyltransferase
• UniProt Gene Name: LCLAT1
• UniProt Synonym Gene Names: AGPAT8; ALCAT1; LYCAT; 1-AGP acyltransferase 8; 1-AGPAT 8
• UniProt Entry Name: LCLT1_HUMAN

Uniprot Description

AGPAT8: Acyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. Required for establishment of the hematopoietic and endothelial lineages. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Lipid Metabolism - ether lipid; Transferase; Endoplasmic reticulum; Secondary Metabolites Metabolism - limonene and pinene degradation; EC 2.3.1.51; Amino Acid Metabolism - tyrosine; Membrane protein, integral; Amino Acid Metabolism - phenylalanine; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 2p23.1

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: phospholipid metabolic process; multicellular organismal development; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; CDP-diacylglycerol biosynthetic process