KLKB1 sirna

KLKB1 siRNA (Human)

Cat. Num. AAA8215399

• Gene Names: KLKB1; PPK; KLK3; PKKD
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KLKB1; KLKB1 siRNA (Human); KLK3; Plasma kallikrein; Fletcher factor; Kininogenin; Plasma prekallikrein; KLKB1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KLKB1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 638

• Applicable Applications for KLKB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KLKB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KLKB1 sirna

siRNA to inhibit KLKB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 78191798
• NCBI GeneID: 3818
• NCBI Accession #: NP_000883.2
• NCBI GenBank Nucleotide #: NM_000892.3
• UniProt Accession #: P03952; Q17RE8; Q17RE9; Q4W5C3; A6NH96; B2R8H9
• Molecular Weight: 71,370 Da
• NCBI Official Full Name: plasma kallikrein preproprotein
• NCBI Official Synonym Full Names: kallikrein B, plasma (Fletcher factor) 1
• NCBI Official Symbol: KLKB1
• NCBI Official Synonym Symbols: PPK; KLK3; PKKD
• NCBI Protein Information: plasma kallikrein
• UniProt Protein Name: Plasma kallikrein
• Protein Family: Plasma kallikrein
• UniProt Gene Name: KLKB1
• UniProt Synonym Gene Names: KLK3
• UniProt Entry Name: KLKB1_HUMAN

NCBI Description

Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. [provided by RefSeq, Jul 2008]

Uniprot Description

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.

Protein type: EC 3.4.21.34; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; fibrinolysis; extracellular matrix organization and biogenesis; positive regulation of fibrinolysis; zymogen activation; blood coagulation; proteolysis; blood coagulation, intrinsic pathway; Factor XII activation; plasminogen activation

Disease: Prekallikrein Deficiency