KIF1A sirna

KIF1A siRNA (Human)

Cat. Num. AAA8221647

• Gene Names: KIF1A; ATSV; MRD9; HSN2C; SPG30; UNC104; C2orf20
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KIF1A; KIF1A siRNA (Human); ATSV; C2orf20; Kinesin-like protein KIF1A; Axonal transporter of synaptic vesicles; Microtubule-based motor KIF1A; Unc-104- and KIF1A-related protein; hUnc-104; KIF1A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KIF1A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1791

• Applicable Applications for KIF1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KIF1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KIF1A sirna

siRNA to inhibit KIF1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 345842524
• NCBI GeneID: 547
• NCBI Accession #: NP_001230937.1
• NCBI GenBank Nucleotide #: NM_001244008.1
• UniProt Accession #: Q12756; O95068; Q13355; Q14752; Q2NKJ6; Q4LE42; Q53T78; Q59GH1; Q63Z40; Q6P1R9; B0I1S5; F5H045
• Molecular Weight: 202,059 Da
• NCBI Official Full Name: kinesin-like protein KIF1A isoform 1
• NCBI Official Synonym Full Names: kinesin family member 1A
• NCBI Official Symbol: KIF1A
• NCBI Official Synonym Symbols: ATSV; MRD9; HSN2C; SPG30; UNC104; C2orf20
• NCBI Protein Information: kinesin-like protein KIF1A
• UniProt Protein Name: Kinesin-like protein KIF1A
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF1A
• UniProt Synonym Gene Names: ATSV; C2orf20; hUnc-104
• UniProt Entry Name: KIF1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the kinesin family and functions as an anterograde motor protein that transports membranous organelles along axonal microtubules. Mutations at this locus have been associated with spastic paraplegia-30 and hereditary sensory neuropathy IIC. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Apr 2012]

Uniprot Description

KIF1A: a member of the kinesin motor protein family. Motor for anterograde axonal transport of synaptic vesicle precursors. Interacts with PPFIA1 and PPFIA4. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: microtubule; kinesin complex; neuron projection; cell soma; cytoplasm

Molecular Function: plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; motor activity; ATP binding

Biological Process: vesicle-mediated transport; metabolic process; cytoskeleton-dependent intracellular transport; anterograde axon cargo transport; microtubule-based movement

Disease: Neuropathy, Hereditary Sensory, Type Iic; Neuropathy, Hereditary Sensory And Autonomic, Type Iia; Spastic Paraplegia 30, Autosomal Recessive; Mental Retardation, Autosomal Dominant 9