KDM2B sirna

KDM2B siRNA (Human)

Cat. Num. AAA829459

• Gene Names: KDM2B; CXXC2; Fbl10; PCCX2; FBXL10; JHDM1B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KDM2B; KDM2B siRNA (Human); CXXC2; FBL10; FBXL10; JHDM1B; PCCX2; Lysine-specific demethylase 2B; CXXC-type zinc finger protein 2; F-box and leucine-rich repeat protein 10; F-box protein FBL10; F-box/LRR-repeat protein 10; JmjC domain-containing histone demethylation protein 1B; Jumonji domain-containing EMSY-interactor methyltransferase motif protein; Protein JEMMA; Protein-containing CXXC domain 2; [Histone-H3]-lysine-36 demethylase 1B; KDM2B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KDM2B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1265

• Applicable Applications for KDM2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KDM2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KDM2B sirna

siRNA to inhibit KDM2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 54112380
• NCBI GeneID: 84678
• NCBI Accession #: NP_001005366.1
• NCBI GenBank Nucleotide #: NM_001005366.1
• UniProt Accession #: Q8NHM5; Q1RLM7; Q8NCI2; Q96HC7; Q96SL0; Q96T03; Q9NS96; Q9UF75; A8MRS1
• Molecular Weight: 63,630 Da
• NCBI Official Full Name: lysine-specific demethylase 2B isoform b
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 2B
• NCBI Official Symbol: KDM2B
• NCBI Official Synonym Symbols: CXXC2; Fbl10; PCCX2; FBXL10; JHDM1B
• NCBI Protein Information: lysine-specific demethylase 2B
• UniProt Protein Name: Lysine-specific demethylase 2B
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM2B
• UniProt Synonym Gene Names: CXXC2; FBL10; FBXL10; JHDM1B; PCCX2; Protein JEMMA
• UniProt Entry Name: KDM2B_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXL10: Histone demethylase that demethylates 'Lys-4' and 'Lys- 36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Belongs to the JHDM1 histone demethylase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Demethylase; EC 1.14.11.27; DNA-binding; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; nucleolus; PcG protein complex; nucleus

Molecular Function: rRNA binding; protein binding; histone demethylase activity; DNA binding; zinc ion binding; histone demethylase activity (H3-K36 specific)

Biological Process: midbrain-hindbrain boundary morphogenesis; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; fourth ventricle development; negative regulation of transcription from RNA polymerase II promoter; third ventricle development; initiation of neural tube closure; histone demethylation; forebrain development; midbrain development; spermatogenesis; negative regulation of neuron apoptosis; embryonic camera-type eye morphogenesis; lateral ventricle development; hindbrain development