KCNJ4 sirna

KCNJ4 siRNA (Human)

Cat. Num. AAA8209150

• Gene Names: KCNJ4; HIR; HRK1; IRK3; HIRK2; IRK-3; Kir2.3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNJ4; KCNJ4 siRNA (Human); IRK3; Inward rectifier potassium channel 4; HIRK2; HRK1; Hippocampal inward rectifier; HIR; Inward rectifier K(+) channel Kir2.3; IRK-3; Potassium channel. inwardly rectifying subfamily J member 4; KCNJ4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KCNJ4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 445

• Applicable Applications for KCNJ4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KCNJ4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNJ4 sirna

siRNA to inhibit KCNJ4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4826798
• NCBI GeneID: 3761
• NCBI Accession #: NP_004972.1
• NCBI GenBank Nucleotide #: NM_004981.1
• UniProt Accession #: P48050; Q14D44
• Molecular Weight: 49,500 Da
• NCBI Official Full Name: inward rectifier potassium channel 4
• NCBI Official Synonym Full Names: potassium channel, inwardly rectifying subfamily J, member 4
• NCBI Official Symbol: KCNJ4
• NCBI Official Synonym Symbols: HIR; HRK1; IRK3; HIRK2; IRK-3; Kir2.3
• NCBI Protein Information: inward rectifier potassium channel 4
• UniProt Protein Name: Inward rectifier potassium channel 4
• Protein Family: Inward rectifier potassium channel
• UniProt Gene Name: KCNJ4
• UniProt Synonym Gene Names: IRK3; HIR; IRK-3
• UniProt Entry Name: KCNJ4_HUMAN

NCBI Description

Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HIR: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily.

Protein type: Membrane protein, integral; Channel, potassium; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: postsynaptic membrane; voltage-gated potassium channel complex; cytoplasmic vesicle membrane; basolateral plasma membrane; plasma membrane; cell junction

Molecular Function: protein binding; inward rectifier potassium channel activity; PDZ domain binding

Biological Process: synaptic transmission; potassium ion import; potassium ion transport