KCNJ12 sirna

KCNJ12 siRNA (Human)

Cat. Num. AAA8227580

• Gene Names: KCNJ12; IRK2; hIRK; IRK-2; hIRK1; KCNJN1; Kir2.2; Kir2.2v; kcnj12x; hkir2.2x
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNJ12; KCNJ12 siRNA (Human); IRK2; KCNJN1; ATP-sensitive inward rectifier potassium channel 12; Inward rectifier K(+) channel Kir2.2; IRK-2; Inward rectifier K(+) channel Kir2.2v; Potassium channel. inwardly rectifying subfamily J member 12; KCNJ12 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KCNJ12 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 433

• Applicable Applications for KCNJ12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KCNJ12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNJ12 sirna

siRNA to inhibit KCNJ12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 23110982
• NCBI GeneID: 3768
• NCBI Accession #: NP_066292.2
• NCBI GenBank Nucleotide #: NM_021012.4
• UniProt Accession #: Q14500; O43401; Q15756; Q8NG63
• Molecular Weight: 49,001 Da
• NCBI Official Full Name: ATP-sensitive inward rectifier potassium channel 12
• NCBI Official Synonym Full Names: potassium channel, inwardly rectifying subfamily J, member 12
• NCBI Official Symbol: KCNJ12
• NCBI Official Synonym Symbols: IRK2; hIRK; IRK-2; hIRK1; KCNJN1; Kir2.2; Kir2.2v; kcnj12x; hkir2.2x
• NCBI Protein Information: ATP-sensitive inward rectifier potassium channel 12
• UniProt Protein Name: ATP-sensitive inward rectifier potassium channel 12
• Protein Family: ATP-sensitive inward rectifier potassium channel
• UniProt Gene Name: KCNJ12
• UniProt Synonym Gene Names: IRK2; KCNJN1; IRK-2
• UniProt Entry Name: KCJ12_HUMAN

NCBI Description

This gene encodes an inwardly rectifying K+ channel which may be blocked by divalent cations. This protein is thought to be one of multiple inwardly rectifying channels which contribute to the cardiac inward rectifier current (IK1). The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

KCNJ12: Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ12 subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: integral to plasma membrane; plasma membrane; intrinsic to membrane

Molecular Function: inward rectifier potassium channel activity

Biological Process: synaptic transmission; potassium ion import; muscle contraction; potassium ion transport; regulation of heart contraction; protein homotetramerization