KCNH1 sirna

KCNH1 siRNA (Human)

Cat. Num. AAA8202821

• Gene Names: KCNH1; EAG; EAG1; TMBTS; h-eag; Kv10.1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNH1; KCNH1 siRNA (Human); EAG; EAG1; Potassium voltage-gated channel subfamily H member 1; Ether-a-go-go potassium channel 1; EAG channel 1; h-eag; hEAG1; Voltage-gated potassium channel subunit Kv10.1; KCNH1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KCNH1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 962

• Applicable Applications for KCNH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KCNH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNH1 sirna

siRNA to inhibit KCNH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4504831
• NCBI GeneID: 3756
• NCBI Accession #: NP_002229.1
• NCBI GenBank Nucleotide #: NM_002238.3
• UniProt Accession #: O95259; O76035; Q14CL3; B1AQ26
• Molecular Weight: 108,597 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily H member 1 isoform 2
• NCBI Official Synonym Full Names: potassium channel, voltage gated eag related subfamily H, member 1
• NCBI Official Symbol: KCNH1
• NCBI Official Synonym Symbols: EAG; EAG1; TMBTS; h-eag; Kv10.1
• NCBI Protein Information: potassium voltage-gated channel subfamily H member 1
• UniProt Protein Name: Potassium voltage-gated channel subfamily H member 1
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: KCNH1
• UniProt Synonym Gene Names: EAG; EAG1; EAG channel 1; h-eag; hEAG1
• UniProt Entry Name: KCNH1_HUMAN

NCBI Description

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Kv10.1: Pore-forming (alpha) subunit of voltage-gated non- inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts. Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv10.1/KCNH1 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32.2

Cellular Component: nucleoplasm; voltage-gated potassium channel complex; integral to plasma membrane; early endosome membrane; cytoplasm; plasma membrane; nuclear inner membrane

Molecular Function: calmodulin binding; protein binding; cyclic nucleotide binding; delayed rectifier potassium channel activity; two-component sensor activity

Biological Process: synaptic transmission; regulation of membrane potential; two-component signal transduction system (phosphorelay); potassium ion transport; myoblast fusion; regulation of cell proliferation

Disease: Zimmermann-laband Syndrome 1; Temple-baraitser Syndrome